Instrument for measuring fluorescence resonance energy transfer

• US 5,911,952 A
• Filed: 04/24/1998
• Issued: 06/15/1999
• Est. Priority Date: 02/16/1994
• Status: Expired due to Fees

• Current Assignee: Hamamatsu Photonics KK
• Original Assignee: Hamamatsu Photonics KK
• Inventors: Tsuji, Akihiko
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/065,585
• Time in Patent Office: 417 Days
• Field of Search: 422/82.08, 422/82.07, 436/172, 250/458.1, 250/461.1, 250/461.2
• US Class Current: 422/82.08
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A plurality of light emission molecules, having different light emission lifetimes, are irradiated with excitation light so that the light emission molecules emit light. The emitted light is divided into at least two different wavelength regions. Temporal changes in each of the at least two wavelength regions of the light emission is measured over at least two different time periods. Information on energy transfer generated between the plurality of light emission molecules is determined based on the measured results in each of the wavelength regions over each of the time periods.

Claims

1. A device for measuring energy transfer between donor fluorophores and acceptor fluorophores under energy-transfer condition included in a sample, the sample also including donor fluorophores and acceptor fluorophores under non-energy-transfer condition, the device comprising:

a light source for irradiating, with excitation light, a sample including donor fluorophores and acceptor fluorophores both under non-energy-transfer condition and under energy-transfer condition, so that the donor fluorophores and the acceptor fluorophores emit fluorescence, the donor fluorophores and the acceptor fluorophores emitting fluorescence of different wavelength bands which are partly overlapped with each other, the donor fluorophores having a fluorescence lifetime longer than a fluorescence lifetime of the acceptor fluorophores,

wherein intensity of fluorescence from the acceptor fluorophores attenuates over time after the irradiation of the excitation light, in accordance with its fluorescent lifetime, the attenuation occurring so that the intensity of fluorescence from the acceptor fluorophores is below a predetermined threshold at a first timing, intensity of fluorescence from the donor fluorophores attenuates over time after the irradiation of the excitation light, in accordance with its fluorescent lifetime, the attenuation occurring so that the intensity of fluorescence from the donor fluorophores is below a second predetermined threshold at a second timing, the fluorescence lifetime of the donor fluorophores varies when energy transfer occurs from the donor fluorophores to the acceptor fluorophores so that the intensity of fluorescence from the donor fluorophores attenuates over time when the energy transfer occurs after the irradiation of the excitation light, in accordance with its varied fluorescent lifetime, the attenuation occurring so that the intensity of fluorescence from the donor fluorophores is below a third predetermined threshold at a third timing different from the second timing;

a light dividing element for dividing light, emitted from the sample, into first light of a first wavelength and second light of a second wavelength, the first wavelength region being only within the fluorescence wavelength band of the donor fluorophores, the second wavelength region being not only within the fluorescence wavelength band of the acceptor fluorophores but also within a predetermined amount of a part of the fluorescence wavelength band of the donor fluorophores;

an optical detector for accumulating an intensity of the first light and an intensity of the second light over a first time period, which is determined between the first timing and the third timing, to thereby obtain a total intensity I_D1 of the first light over the first time period and a total intensity I_A1 of the second light over the first time period, and for accumulating an intensity of the first light and an intensity of the second light over a second time period, which is determined between the third timing and the second timing, to thereby obtain a total intensity I_D2 of the first light over the second time period and a total intensity I_A2 of the second light over the second time period; and

a data processing portion for calculating the following formula;

space="preserve" listing-type="equation">{(I.sub.D2 /I.sub.D1)-(I.sub.A2 /I.sub.A1)}/(I.sub.D2 /I.sub.D1)

to thereby determine energy transfer occurring between the donor fluorophores and the acceptor fluorophores under energy-transfer condition in the sample.

2. A device as claimed in claim 1, wherein the optical detector includes:

a first light detector located capable of receiving the first light, the first light detector having a gate;

a second light detector located capable of receiving the second light, the second light detector having a gate;

a controller for controlling the gates of the first and second light detectors to open during the first time period, the gate-opened first and second light detectors detecting the intensities of the first light and the second light over the first time period, the intensities of the first and second lights detected during the first time period being integrated over the first time period into the total intensity amounts I_D1 and I_A1, and for controlling the gates of the first and second light detectors to open during the second time period, the gate-opened first and second light detectors detecting the intensities of the first light and the second light over the second time period, the intensities of the first and second lights detected during the second time period being integrated over the second time period into the total intensity amounts I_D2 and I_A2.

3. A device as claimed in claim 2, wherein the optical detector further includes:

a first converter for converting the intensities of the first light detected during the first time period and the second time period into first and third electric signals;

a first calculation portion for integrating the first electric signals over the first time period into the amount I_D1 and for integrating the third electric signals over the second time period into the amount I_D2 ;

a second converter for converting the intensities of the second light detected during the first time period and the second time period into second and fourth electric signals; and

a second calculation portion for integrating the second electric signals over the first time period into the amount I_A1 and for integrating the fourth electric signals over the second time period into the amount I_A2.

4. A device as claimed in claim 1, wherein the first light detector includes a first image intensifier, and the second light detector includes a second image intensifier, and

wherein the controller inputs trigger signals to the first and second image intensifiers during the first time period, the triggered first and second image intensifiers multiplying the intensities of the first light and the second light over the first time period, the intensities of the first and second lights multiplied during the first time period being integrated over the first time period into the total intensity amounts I_D1 and I_A1, and inputs trigger signals to the first and second image intensifiers during the second time period, the triggered first and second image intensifiers multiplying the intensities of the first light and the second light over the second time period, the intensities of the first and second lights multiplied during the second time period being integrated over the second time period into the total intensity amounts I_D2 and I_A2.

5. A device as claimed in claim 4, wherein the optical detector further includes:

a first CCD camera for picking up the first light, with its intensity being multiplied during the first time period, and for converting the first light into first electric signals, the first electric signals being integrated over the first time period into the amount I_D1, the first CCD camera further picking up the first light, with its intensity being multiplied during the second time period, and converting the first light into third electric signals, the third electric signals being integrated over the second time period into the amount I_D2 ; and

a second CCD camera for picking up the second light, with its intensity being multiplied during the first time period, and for converting the first light into second electric signals, the second electric signals being integrated over the first time period into the amount I_A1, the second CCD camera further picking up the second light, with its intensity being multiplied during the second time period, and converting the second light into fourth electric signals, the fourth electric signals being integrated over the second time period into the amount I_A2.

6. A device as claimed in claim 5, wherein the first CCD camera has a plurality of first pixels each for receiving a corresponding part of the first light which is multiplied during the first time period and for converting the received light into a first electric charge, each first pixel again receiving a corresponding part of the first light which is multiplied during the second time period and converting the received light into a third electric charge, and

wherein the second CCD camera has a plurality of second pixels each for receiving a corresponding part of the second light which is multiplied during the first time period and for converting the received light into a second electric charge, each second pixel again receiving a corresponding part of the second light which is multiplied during the second time period and converting the received light into a fourth electric charge.

7. A device as claimed in claim 6, wherein the optical detector further includes:

a first frame memory connected to the first CCD camera for receiving the first electric charge produced from each first pixel and for accumulating the received first electric charge, and for receiving the third electric charge produced from each first pixel and for accumulating the received third electric charge;

a first calculation device for spatially integrating the first electric charges supplied from all the first pixels to obtain the amount I_D1 and for spatially integrating the third electric charges supplied from all the first pixels to obtain the amount I_D2 ;

a second frame memory connected to the second CCD camera for receiving the second electric charge produced from each second pixel and for accumulating the received second electric charge, and for receiving the fourth electric charge produced from each second pixel and for accumulating the received fourth electric charge;

a second calculation device for spatially integrating the second electric charges from all the second pixels to obtain the amount I_A1 and for spatially integrating the fourth electric charges supplied from all the second pixels to obtain the amount I_A2.

8. A device as claimed in claim 7, wherein the optical detector includes:

a single streak tube located capable of receiving the first light and the second light at different regions along a first direction; and

a voltage controller for inputting a first sweep voltage to the streak tube during the first time period, the first sweep voltage-driven streak tube introducing the first light and the second light into first and second positions arranged along first direction, the intensities of the first and second lights thus introduced into the first and second positions being integrated into the amounts I_D1 and I_A1, the voltage controller inputting a second sweep voltage to the streak tube during the second time period, the second sweep voltage-driven streak tube introducing the first light and the second light into third and fourth positions arranged in the first direction, the third and fourth positions being shifted from the first and second positions along a second direction substantially perpendicular to the first direction, the intensities of the first and second lights thus introduced into the third and fourth positions being integrated into the amounts I_D2 and I_A2.

9. A device as claimed in claim 8, wherein the optical detector further includes a single CCD camera for picking up the lights introduced into the first, second, third, and fourth positions and for converting the lights into first, second, third, and fourth electric signals, the first, second, third, and fourth electric signals being integrated into the amounts I_D1, I_A1, I_D2, and I_A2, respectively.

10. A device as claimed in claim 1, wherein the light dividing element includes a filter for dividing the light, emitted from the sample, into the first light and the second light .

11. A device as claimed in claim 1, wherein the light dividing element includes a diffraction grating for dividing the light, emitted from the sample, into the first light and the second light.

12. A device as claimed in claim 1, wherein the light, emitted from the sample, is divided in a fist direction into the first light and the second light,

wherein a single streak tube is inputted with a first sweep voltage during the first time period to introduce the received first and second lights into first and second positions arranged in the first direction,

wherein the streak camera is inputted with a second sweep voltage during the second time period to introduce the received first and second lights into third and fourth positions arranged in the first direction, the third and fourth positions being shifted from the first and second positions along a second direction almost perpendicular to the first direction,

wherein a single CCD camera connected to the streak tube receives the lights introduced to the first, second, third, and fourth positions and produces first, second, third, and fourth electric signals accordingly, and

wherein the first, second, third, and fourth electric signals are integrated into the amounts I_D1, I_A1, I_D2, and I_A2.