Materials and methods for the immobilization of bioactive species onto polymeric substrates
First Claim
1. A material having immobilized bioactive species comprising:
- a support member;
a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the Support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-liked together to itself in situ;
a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and
at least one type of bioactive species attached to the second layer.
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Accused Products
Abstract
The present invention is directed to support members having surfaces that are rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto the surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on the support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member, as well as, to provide a substrate for immobilizing bioactive species thereto. Methods for forming the first and second layers are also provided. In addition methods for attaching bioactive substances to the hydrophilic polymers are provided.
376 Citations
89 Claims
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1. A material having immobilized bioactive species comprising:
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a support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the Support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-liked together to itself in situ; a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76)
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18. A material having immobilized bioactive species comprising:
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a support member comprised of a polytetrafluoroetylene material; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
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27. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; a second layer comprised of at least one species of a hydrophilic polymer covalently attached to the first layer; and at least one type of bioactive species attached to the second layer. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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38. A material having immobilized bioactive species comprising:
a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-linked to itself in situ; and
at least one type of bioactive species attached to the first layer.- View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A material having immobilized bioactive species comprising:
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a support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member, wherein the polymeric surfactant is chemically different than the support member, and wherein the polymeric surfactant is cross-linked to itself in situ; a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer; and at least one type of bioactive species attached to at least one layer of the plurality of layers. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56)
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57. A method of making a material having immobilized bioactive species which comprises:
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providing a hydrophobic support member; attaching a first layer comprised of polymeric surfactants to the support member non-covalently; cross-linking the polymeric surfactants in situ with a cross-linking agent; attaching a second layer comprised of at least one type of hydrophilic polymer to the first layer convalently; and attaching a bioactive species to the second layer. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66)
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77. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; and at least one type of bioactive species attached to the first layer. - View Dependent Claims (78, 79, 80, 81, 82)
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83. A material having immobilized bioactive species comprising:
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a hydrophobic support member; a first layer comprised of at least one species of a polymeric surfactant non-covalently attached to the support member and cross-linked to itself in situ; a plurality of layers attached to one another wherein each layer is comprised of at least one species of a hydrophilic polymer and wherein at least one layer of the plurality of layers is covalently attached to the first layer; and at least one type of bioactive species attached to at least one layer of the plurality of layers. - View Dependent Claims (84, 85, 86, 87, 88, 89)
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Specification