Unitary one-piece sample mini-column

• US 5,918,273 A
• Filed: 02/20/1997
• Issued: 06/29/1999
• Est. Priority Date: 07/09/1996
• Status: Expired due to Fees

• Current Assignee: Amersham Pharmacia Biotech AB
• Original Assignee: Amersham Pharmacia Biotech AB
• Inventors: Horn, Marcus J.
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US08/802,511
• Time in Patent Office: 859 Days
• Field of Search: 73/61.55, 422/67, 422/70, 141/130
• US Class Current: 73/61.55
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/8804 automated systems
• CPC Class Codes: G01N 2030/8831 involving peptides or proteins
• CPC Class Codes: G01N 2030/8881 Modular construction, specially adapted therefor
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 30/24 Automatic injection systems
• CPC Class Codes: G01N 30/36 in high pressure liquid systems
• CPC Class Codes: G01N 30/466 with separation columns in parallel
• CPC Class Codes: G01N 30/6043 in parallel
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

This invention is directed to a unitary one-piece sample mini-column for a cassette chemical immobilization and treatment system for enabling the performance of various complex chemistries. The cassette assembly comprises a plurality of analyte sample columns ("mini-columns"), reagent wells containing pre-packaged reagents including powdered, microencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment mechanisms for the analyte sample columns, and a machine readable instruction code set for identfying a chemical treatment protocol. The mini-columns are improved columns having a high pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. The same chemistries may be performed at all sample mini-column addresses, or a separate, completely independent set of protocols may be defined for each address, or for each block of addresses pursuant to the instructions contained in the machine readable code on the cassette.

Claims

1. A unitary, one-piece mini-column for retaining a chemical sample for chemical treatment, said mini-column comprising a centrally disposed chamber extending longitudinally through a cylindrical column, said chamber terminating into a first open end, said first open end having a flared, tapered inlet port, said first open end enabling slip fit, flow-through, physical connection with a connection sleeve, said connection sleeve extending from a through-hole in the tapered, conical end of a sampling funnel, said connection resulting in smooth, continuous transition of a taper from said conical end of said funnel to the taper of said inlet port, said chamber terminating into a second open end, said second open end having a flared, tapered outlet port, said outlet port being in flow-through communication with said inlet port, said tapered inlet port and outlet port each providing localized, leakproof communication with a compression fit interface means.

2. A mini-column as in claim 1 further comprising a solid support material disposed in the central chamber, said solid support material being treated to immobilize said chemical sample, said solid support material being retained in said mini-column by porous frits disposed in said central chamber.

3. A one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber extending longitudinally through a cylindrical column, said chamber terminating into a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in the end of a sampling funnel, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating into a second open end, said second open end having an outlet port, said outlet port being in communication with said inlet port, wherein said one-piece column defines at least a single bevel surface.

4. A one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber extending longitudinally through a cylindrical column, said chamber terminating into a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in the end of a sampling funnel, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating into a second open end, said second open end having an outlet port, said outlet port being in communication with said inlet port, wherein said inlet port defines a bevel sealing surface.

5. An arrangement for holding chemical samples, including a funnel and a one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber having a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in said funnel, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating in a second open end, said second open end having an outlet port, said outlet port being in communication with said inlet port, wherein said inlet port defines a bevel sealing surface.

6. An arrangement for holding chemical samples, including a funnel and a one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber having a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in said funnel, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating in a second open end, said second open end having an outlet port, said outlet port being in communication with said inlet port, further comprising first and second bevel surfaces.

7. The arrangement according to claim 6 wherein said first bevel surface is adapted for sealing.

8. The arrangement according to claim 6 wherein said second bevel surface is adapted for sealing.