NADH dehydrogenase subunits

• US 5,919,686 A
• Filed: 08/08/1997
• Issued: 07/06/1999
• Est. Priority Date: 08/08/1997
• Status: Expired due to Fees

• Current Assignee: Incyte Pharmaceuticals, Inc.
• Original Assignee: Incyte Pharmaceuticals, Inc.
• Inventors: Hillman, Jennifer L., Guegler, Karl J., Bandman, Olga, Shah, Purvi
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Tung, Peter P.
• Application Number:
  US08/907,706
• Time in Patent Office: 697 Days
• Field of Search: 435/69.1, 435/325, 435/6, 435/320.1, 435/191, 536/23.1, 536/23.2
• US Class Current: 435/191
• CPC Class Codes: C12N 9/0036 acting on NADH or NADPH (1.6)
• CPC Class Codes: C12Y 106/05003 NADH dehydrogenase (ubiquinone) (1.6.5.3)

Abstract

The invention provides two human NADH dehydrogenase subunits (HNDS) and polynucleotides which identify and encode HNDS. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of HNDS.

Claims

1. An isolated and purified polynucleotide encoding the NADH dehydrogenase subunit having the amino acid sequence of SEQ ID NO:

1.

2. A composition comprising the polynucleotide sequence of claim 1.

3. A polynucleotide which is fully complementary to the polynucleotide of claim 1.

11. An expression vector containing the polynucleotide of claim 1.

13. A host cell containing the vector of claim 11.

15. A method for producing a polypeptide having the amino acid sequence of SEQ ID NO:

1, the method comprising the steps of;

a) culturing the host cell of claim 13 under conditions suitable for the expression of the polypeptide; and

b) recovering the polypeptide from the host cell culture.

17. A method for detecting a polynucleotide which encodes NADH dehydrogenase subunit in a biological sample comprising the steps of:

a) hybridizing the polynucleotide of claim 3 to nucleic acid material of a biological sample, thereby forming a hybridization complex; and

b) detecting said hybridization complex, wherein the presence of said complex correlates with the presence of a polynucleotide encoding NADH dehydrogenase subunit in said biological sample.

18. The method of claim 17 wherein the nucleic acid material is amplified by the polymerase chain reaction prior to hybridization.

4. An isolated and purified polynucleotide having the sequence of SEQ ID NO:

2.

5. A polynucleotide which is fully complementary to the polynucleotide of claim 4.

6. An isolated and purified polynucleotide encoding the NADH dehydrogenase subunit having the amino acid sequence of SEQ ID NO:

3.

7. A composition comprising the polynucleotide sequence of claim 6.

8. A polynucleotide which is fully complementary to the polynucleotide of claim 6.

12. An expression vector containing the polynucleotide of claim 6.

14. A host cell containing the vector of claim 12.

16. A method for producing a polypeptide having the amino acid sequence of SEQ ID NO:

3, the method comprising the steps of;

a) culturing the host cell of claim 14 under conditions suitable for the expression of the polypeptide; and

b) recovering the polypeptide from the host cell culture.

19. A method for detecting a polynucleotide which encodes NADH dehydrogenase subunit in a biological sample comprising the steps of:

a) hybridizing the polynucleotide of claim 8 to nucleic acid material of a biological sample, thereby forming a hybridization complex; and

b) detecting said hybridization complex, wherein the presence of said complex correlates with the presence of a polynucleotide encoding NADH dehydrogenase subunit in said biological sample.

20. The method of claim 19 wherein the nucleic acid material is amplified by the polymerase chain reaction prior to hybridization.

9. An isolated and purified polynucleotide having the sequence of SEQ ID NO:

4.

10. A polynucleotide which is fully complementary to the polynucleotide of claim 9.