Process for controlling contamination of nucleic acid amplification reactions

  • US 5,945,313 A
  • Filed: 11/03/1997
  • Issued: 08/31/1999
  • Est. Priority Date: 06/01/1989
  • Status: Expired due to Term
First Claim
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1. A method for controlling contamination in sequential nucleic acid amplification processes comprising first and second nucleic acid amplification processes to amplify nucleic acid sequences in a first and second sample, respectively, which comprises:

  • (a) carrying out said first nucleic acid amplification process on said nucleic acid sequence in said first sample in the presence of an exo-sample nucleotide, to thereby generate an exo-sample nucleotide-containing product of amplification; and

    (b) subjecting said exo-sample nucleotide-containing product to a treatment which renders said exo-sample nucleotide-containing product substantially unamplifiable andwherein said treatment does not substantially prevent oligonucleotide-dependent amplification of a nucleic acid that does not contain said exo-sample nucleotide prior to carrying out said second amplification process on said nucleic acid in said second sample.

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