Disposable glucose test strip and method and compositions for making same

• US 5,951,836 A
• Filed: 01/12/1998
• Issued: 09/14/1999
• Est. Priority Date: 02/14/1996
• Status: Expired due to Term

• Current Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Original Assignee: Selfcare Inc. (Johnson & Johnson)
• Inventors: Scott, David, Alvarez-Icaza, Manuel, Hall, Geoff, Plotkin, Elliot V., McAleer, Jerome F.
• Primary Examiner(s): Bell, Bruce F.
• Application Number:
  US09/005,710
• Time in Patent Office: 610 Days
• Field of Search: 204/403, 204/415, 204/418, 435/287.1, 435/289.1, 435/817, 435/4, 435/14
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 33/5002 Partitioning blood components
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An improved disposable glucose test strip for use in a test meter of the type which receives a disposable test strip and a sample of blood from a patient and performs an electrochemical analysis is made using a working formulation containing a filler, an enzyme effective to oxidize glucose, e.g., glucose oxidase, and a mediator effective to transfer electrons from the enzyme. The working formulation is printed over a conductive carbon base layer to form a working electrode. The filler, for example a silica filler, is selected to have a balance of hydrophobicity and hydrophilicity such that one drying it forms a two-dimensional network on the surface of the conductive base layer. The response of this test strip is essentially temperature independent over relevant temperature ranges and is substantially insensitive to the hematocrit of the patient.

Claims

1. An aqueous composition comprising a binder, a filler having both hydrophobic and hydrophilic surface regions, at least one of an enzyme effective to oxidize glucose and an electron transfer mediator.

2. The composition of claim 1, wherein the filler is non-conductive.