Biosensor
First Claim
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1. A biosensor comprising:
- an electrically insulating base plate,an electrode system including a working electrode and a counter electrode provided on said base plate, anda reaction layer containing a hydrophilic polymer, 0.5 to 50 μ
g of an enzyme and an electron acceptor formed over said electrode system,wherein said reaction layer contains said hydrophilic polymer in a range of 150 to 1,000% by weight of said enzyme,wherein said reaction layer is dissolved in a sample solution comprising an analyte dissolved in an aqueous media prior to detection of a reaction between said enzyme and said analyte, andwherein said reaction layer is essentially soluble in said sample solution.
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Abstract
Disclosed is a high performance biosensor free from the effect of any components existing in a sample solution other than a substrate. The biosensor comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on the base plate, and a reaction layer containing a hydrophilic polymer, an enzyme and an electron acceptor located over the electrode system. The reaction layer contains an excess amount of hydrophilic polymer in a range of 150 to 1,000% by weight of the enzyme.
208 Citations
6 Claims
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1. A biosensor comprising:
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an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on said base plate, and a reaction layer containing a hydrophilic polymer, 0.5 to 50 μ
g of an enzyme and an electron acceptor formed over said electrode system,wherein said reaction layer contains said hydrophilic polymer in a range of 150 to 1,000% by weight of said enzyme, wherein said reaction layer is dissolved in a sample solution comprising an analyte dissolved in an aqueous media prior to detection of a reaction between said enzyme and said analyte, and wherein said reaction layer is essentially soluble in said sample solution. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification