Method of extracting selected sweet glycosides from the Stevia rebaudiana plant

• US 5,962,678 A
• Filed: 02/06/1998
• Issued: 10/05/1999
• Est. Priority Date: 09/13/1996
• Status: Expired due to Fees

• Current Assignee: Alberta Research Council
• Original Assignee: Alberta Research Council
• Inventors: Ippolito, Robert Maurice, Payzant, John Donald, Laidler, James Kenneth
• Primary Examiner(s): Lee, Howard C.
• Application Number:
  US09/019,558
• Time in Patent Office: 606 Days
• Field of Search: 424/195.1, 514/783, 536/4.1, 536/5, 536/6.3, 536/18.1, 536/127, 536/128
• US Class Current: 536/128
• CPC Class Codes: A23L 27/36 Terpene glycosides

Abstract

Individual sweet glycosides are obtained from the stevia rebaudiana plant. A mixture of sweet glycosides extracted from the stevia rebaudiana plant are processed to remove impurities by using two ion exchange columns. After removing the mixed sweet glycosides from the second column with methanol the solution is dried. Upon refluxing the dried solids in a methanol solution and then cooling the solution, Stevioside precipitates out. The filtrate is further concentrated and cooled to precipitate out Rebaudioside A. This Rebaudioside A can be further purified as can the previously obtained Stevioside.

Claims

1. A method of extracting Rebaudioside A from a mixture of Rebaudioside A and Stevioside obtained from the Stevia rebaudiana plant comprising the steps of:

a) treating the Stevia rebaudiana plant to extract an aqueous liquid solution containing mixed sweet glycosides and non-sweet glycosides;

b) separating the non-sweet glycosides from the mixed sweet glycosides and drying the mixed sweet glycosides;

c) dissolving the mixed sweet glycosides obtained in step (b) with solvent selected from the group consisting of methanol or methanol-water solution to form a solution, refluxing the solution and then cooling the solution to precipitate Stevioside;

d) filtering the solution to recover the precipitate Stevioside and a filtrate containing other sweet glycosides;

e) concentrating the filtrate from step (d) by heating and then cooling the solution to precipitate Rebaudioside A;

f) dissolving the precipitate Rebaudioside A in a solvent selected from the group consisting of methanol or methanol-water solution to form a solution, refluxing the solution and then cooling the solution to precipitate the Rebaudioside A at a higher purity; and

g) recovering the higher purity Rebaudioside A.

2. A method according to claim 1, wherein the separating of the non-sweet glycosides from the mixed sweet glycosides in step (b) comprises the steps ofpassing the mixture of the sweet and non-sweet glycosides through a first resin column which absorbs the non-sweet glycosides and which lets the mixed sweet glycosides pass through;

2) passing the mixed sweet glycosides from step (1) through a second resin column which absorbs the mixed sweet glycosides;

3) eluting the mixed sweet glycosides from the second resin with a water-soluble organic solvent to form a mixture; and

4) spray drying the mixture to obtain the solid mixed sweet glycosides.

3. A method according to claim 1, wherein the solvent in step (c) is anhydrous methanol.

4. A method according to claim 1, further comprising repeating steps (f) and (g) until a final crystallized solid of desired purity of Rebaudioside A is obtained.

5. A method according to claim 4 wherein the desired purity of Rebaudioside A is at least 95%.