Method of extracting selected sweet glycosides from the Stevia rebaudiana plant

  • US 5,962,678 A
  • Filed: 02/06/1998
  • Issued: 10/05/1999
  • Est. Priority Date: 09/13/1996
  • Status: Expired due to Fees
First Claim
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1. A method of extracting Rebaudioside A from a mixture of Rebaudioside A and Stevioside obtained from the Stevia rebaudiana plant comprising the steps of:

  • a) treating the Stevia rebaudiana plant to extract an aqueous liquid solution containing mixed sweet glycosides and non-sweet glycosides;

    b) separating the non-sweet glycosides from the mixed sweet glycosides and drying the mixed sweet glycosides;

    c) dissolving the mixed sweet glycosides obtained in step (b) with solvent selected from the group consisting of methanol or methanol-water solution to form a solution, refluxing the solution and then cooling the solution to precipitate Stevioside;

    d) filtering the solution to recover the precipitate Stevioside and a filtrate containing other sweet glycosides;

    e) concentrating the filtrate from step (d) by heating and then cooling the solution to precipitate Rebaudioside A;

    f) dissolving the precipitate Rebaudioside A in a solvent selected from the group consisting of methanol or methanol-water solution to form a solution, refluxing the solution and then cooling the solution to precipitate the Rebaudioside A at a higher purity; and

    g) recovering the higher purity Rebaudioside A.

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