Process for obtaining DNA, RNA, peptides, polypeptides, or proteins, by recombinant DNA technique

  • US 5,976,862 A
  • Filed: 06/05/1995
  • Issued: 11/02/1999
  • Est. Priority Date: 03/30/1985
  • Status: Expired due to Term
First Claim
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1. A process for the production of a host cell capable of producing a transcription product or a translation product comprising an expression vector, wherein said expression vector comprises at least one stochastic sequence of polynucleotides comprising the steps of:

  • producing a library of expression vectors capable of producing a transcription product or a translation product, said expression vectors comprising at least one stochastic sequence of polynucleotides, said expression vectors being produced by the following steps;

    providing in an appropriate buffer at least three different sequences of oligonucleotides, said oligonucleotides each comprising at least 7 nucleotide residues;

    polymerizing said oligonucleotides in a manner to form a stochastic sequence of polynucleotides;

    ligating said stochastic sequence of polynucleotides into a linearized expression vector; and

    transforming a competent host cell with said ligated expression vector.

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