Gene encoding endo-.beta.-N-acetylglucosaminidase A
First Claim
Patent Images
1. An isolated DNA encoding a polypeptide possessing endo-β
- -N-acetylglucosaminidase A activity, the isolated DNA being selected from the group consisting of;
(a) a DNA having the nucleotide sequence of SEQ ID NO;
2;
(b) a DNA having a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;
1; and
(c) a DNA sequence hybridizing to (a) or (b) under conditions of using a solution containing 6×
SSC, 0.5% SDS, 5×
Denhardt'"'"'s solution and 100 μ
g/ml of salmon sperm DNA, and incubating overnight at 65°
C.
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Abstract
An isolated DNA having a sequence encoding a polypeptide possessing endo-β-N-acetylglucosaminidase A activity or functionally equivalent variants thereof; and a method for producing a polypeptide possessing endo-β-N-acetylglucosaminidase A activity or functionally equivalent variants thereof using the isolated DNA by recombinant DNA technology.
2 Citations
8 Claims
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1. An isolated DNA encoding a polypeptide possessing endo-β
- -N-acetylglucosaminidase A activity, the isolated DNA being selected from the group consisting of;
(a) a DNA having the nucleotide sequence of SEQ ID NO;
2;(b) a DNA having a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;
1; and(c) a DNA sequence hybridizing to (a) or (b) under conditions of using a solution containing 6×
SSC, 0.5% SDS, 5×
Denhardt'"'"'s solution and 100 μ
g/ml of salmon sperm DNA, and incubating overnight at 65°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- -N-acetylglucosaminidase A activity, the isolated DNA being selected from the group consisting of;
Specification