Device for determining an analyte in a sample

• US 5,989,924 A
• Filed: 09/30/1997
• Issued: 11/23/1999
• Est. Priority Date: 09/30/1997
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Lovell, Stephen J., Root, Richard T.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/941,184
• Time in Patent Office: 784 Days
• Field of Search: 422/55-58, 422/61, 435/6, 435/287.1, 435/287.2, 435/287.7, 435/287.8, 435/287.9, 435/810, 436/164, 436/169, 436/514, 436/518, 436/530, 436/805, 436/809, 436/810
• US Class Current: 436/518
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

The present invention relates to a device for determining an analyte in a fluid sample in an assay. The device comprises a lid having a plurality of elements and a membrane. The elements have openings which allow passage of fluid to the membrane. The membrane is supported by the lid and covers the openings of the elements. The undersurface of the membrane is in contact with a porous wick which contains a first zone which includes binder attached to a particulate label and a second zone containing salt. The wick extends downward from the membrane and is in contact with the fluid sample.

Claims

1. An analytical device for determining an analyte in a fluid sample in an assay comprising:

a lid having a plurality of elements, said elements having openings suitable to allow passage of a fluid therethrough;

a membrane comprising membrane indicator areas containing binder for said analyte wherein said membrane is supported by said lid and covers the openings of said elements and wherein the membrane further comprises an under surface which is in contact with a porous wick wherein said wick comprises a top portion which extends through the openings of said elements and a bottom portion which comprises a first zone containing tracer which is comprised of binder for said analyte attached to a particulate label, and a second zone containing a salt to induce hybridization, wherein said bottom portion extends downward from said membrane and is in contact with said analyte.

2. The device according to claim 1 wherein said analyte is the end product of a DNA amplification reaction.

3. The device according to claim 1 wherein said binder attached to a particulate label is comprised of oligonucleotides.

4. The device according to claim 3 wherein said particulate label is a latex bead.

5. The device according to claim 1 wherein said binder in the membrane indicator area comprises oligonucleotides.

6. The device according to claim 1 wherein said salt is sodium chloride.

7. The device according to claim 1 wherein said salt is selected from the group consisting of sodium phosphate, potassium chloride, potassium phosphate and magnesium chloride.

8. An analytical device for determining an analyte in a fluid sample in an assay comprising:

a lid having a plurality of elements, said elements having openings suitable to allow passage of a fluid therethrough;

a membrane comprising membrane indicator areas containing binder for said analyte wherein said membrane is supported by said lid and covers the openings of said elements and wherein the membrane further comprises an under surface which is in contact with a porous wick wherein said wick comprises a top portion which extends through the openings of said elements and a bottom portion which comprises a first zone containing tracer which is comprised of binder for said analyte attached to a particulate label and a second zone containing a salt to induce hybridization, wherein said second zone is within said first zone, and further, wherein said bottom portion extends downward from said membrane and is in contact with said analyte.

9. The device according to claim 8 wherein said analyte is the end product of a DNA amplification reaction.

10. The device according to claim 8 wherein said binder attached to a particulate label is comprised of oligonucleotides.

11. The device according to claim 10 wherein said particulate label is a latex bead.

12. The device according to claim 8 wherein said binder in the membrane indicator area comprises oligonucleotides.

13. The device according to claim 8 wherein said salt is sodium chloride.

14. The device according to claim 8 wherein said salt is selected from the group consisting of sodium phosphate, potassium chloride, potassium phosphate and magnesium chloride.

15. An analytical device for determining an analyte in a fluid sample in an assay comprising:

a lid having a plurality of elements, said elements having openings suitable to allow passage of a fluid therethrough;

a membrane comprising membrane indicator areas containing binder for said analyte wherein said membrane is supported by said lid and covers the openings of said elements and wherein the membrane further comprises an under surface which is in contact with a porous wick wherein said wick comprises a top portion which extends through the openings of said elements and a bottom portion which comprises a first zone containing tracer which is comprised of binder for said analyte attached to a particulate label and a second zone containing a salt to induce hybridization, wherein said first zone is above said second zone, and further wherein said bottom portion extends downward from said membrane and is in contact with said analyte.

16. The device according to claim 15 wherein said analyte is the end product of a DNA amplification reaction.

17. The device according to claim 15 wherein said binder attached to a particulate label is comprised of oligonucleotides.

18. The device according to claim 17 wherein said particulate label is a latex bead.

19. The device according to claim 15 wherein said binder in the membrane indicator area comprises oligonucleotides.

20. The device according to claim 15 wherein said salt is sodium chloride.

21. The device according to claim 15 wherein said salt is selected from the group consisting of sodium phosphate, potassium chloride, potassium phosphate and magnesium chloride.