Stable transformation of plant cells

  • US 5,990,387 A
  • Filed: 10/06/1994
  • Issued: 11/23/1999
  • Est. Priority Date: 06/10/1988
  • Status: Expired due to Term
First Claim
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1. A method of producing a fertile, stably transformed Zea mays plant, comprising the steps of:

  • (a) providing a foreign DNA comprising an expression vector carrying a gene of interest;

    (b) providing a Zea mays embryogenic callus;

    (c) introducing the foreign DNA into the embryogenic callus by one or more microparticle bombardments; and

    (d) regenerating the fertile Zea mays plant which is stably transformed with the foreign DNA.

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