Stable transformation of plant cells

• US 5,990,387 A
• Filed: 10/06/1994
• Issued: 11/23/1999
• Est. Priority Date: 06/10/1988
• Status: Expired due to Term

• Current Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Original Assignee: Pioneer Hi-Bred International (Corteva, Inc.)
• Inventors: Weissinger, Arthur, Tomes, Dwight T., Klein, Theodore M., Sanford, John C.
• Primary Examiner(s): Benzion, Gary
• Application Number:
  US08/319,352
• Time in Patent Office: 1,874 Days
• Field of Search: 435/172.1, 435/172.3, 435/240.4, 435/320.1, 435/412, 435/424, 435/430, 435/430.1, 800/205, 800/320.1, 800/278, 800/288, 800/293, 800/300.1, 935/52, 935/53, 935/85
• US Class Current: 800/293
• CPC Class Codes: C12N 15/8207 by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Abstract

The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.

Claims

1. A method of producing a fertile, stably transformed Zea mays plant, comprising the steps of:

(a) providing a foreign DNA comprising an expression vector carrying a gene of interest;

(b) providing a Zea mays embryogenic callus;

(c) introducing the foreign DNA into the embryogenic callus by one or more microparticle bombardments; and

(d) regenerating the fertile Zea mays plant which is stably transformed with the foreign DNA.

2. The method according to claim 1, wherein the embryogenic callus is Type I callus.

3. The method according to claim 1, wherein the embryogenic callus is Type II callus.

4. The method according to claim 1, wherein the gene is a herbicide resistance gene.

5. The method according to claim 1, wherein step (b) further comprises providing the Zea mays embryogenic callus on a solid suport.

6. The method according to claim 1, wherein step (b) further comprises providing the Zea mays embryogenic callus on a liquid medium.

7. The method according to claim 4, wherein the herbicide resistance gene is a chlorsulfuron resistance gene.

8. A method of producing a fertile, stably transformed Zea mays plant, comprising the steps of:

(a) providing a foreign DNA comprising an expression vector carrying a gene of interest;

(b) providing a Zea mays embryogenic suspension culture;

(c) introducing the foreign DNA into the embryogenic suspension culture by one or more microparticle bombardments; and

(d) regenerating the fertile Zea mays plant which is stably transformed with the foreign DNA.

9. The method according to claim 8, wherein the gene is a herbicide resistance gene.

10. The method according to claim 9, wherein the herbicide resistance gene is a chlorsulfuron resistance gene.

11. The method according to claim 8, wherein step (b) further comprises providing the Zea mays embryogenic suspension culture on a solid suport.

12. The method according to claim 8, wherein step (b) further comprises providing the Zea mays embryogenic suspension culture on a liquid medium.