Method for determining nucleotide identity through extension of immobilized primer

  • US 6,004,744 A
  • Filed: 10/11/1991
  • Issued: 12/21/1999
  • Est. Priority Date: 03/05/1991
  • Status: Expired due to Term
First Claim
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1. A reagent composition which comprises an aqueous carrier, a primer nucleic acid molecule, a template nucleic acid of interest hybridized thereto, and an admixture of at least two different terminators of a nucleic acid template-dependent, primer extension reaction, each of the terminators being capable of specifically terminating an extension reaction of said primer hybridized to said template nucleic acid molecule of interest in a manner strictly dependent on the identity of the unpaired nucleotide base in said template nucleic acid molecule of interest immediately adjacent to, and downstream of, the 3'"'"' end of said primer, and at least one of the terminators being labeled with a detectable marker, and wherein said reagent composition lacks dATP, dCTP, dGTP, and dTTP.

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