Method of manufacturing a sensitive single-layer system for measuring the concentration of analytes, and a system produced by this method
First Claim
1. Method of manufacturing a single-layer system for measuring concentration of analytes, comprising:
- adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material;
mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture;
applying the mixture under a pressure of 12 to 20×
104 Pa onto a substrate to obtain a sensitive layer having a thickness and shape;
polymerizing, polycondensing or hardening the matrix material; and
homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator.
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Abstract
Method of manufacturing a sensitive single-layer system for optically detecting a concentration of an analyte, such as, for example, oxygen, the analyte being capable of investigation in liquid or gaseous aggregate condition or in a dissolved condition, by adsorption, luminescence or luminescence quenching of an indicator sensitive to the analyte, which is preferably permanently immobilized in the optical beam path. The sensitive single-layer system achieves a reproducible and extremely short response behavior. Indicators are adsorbed on a filler material and thereafter a mixture is produced with a matrix material permeable to the analyte to be detected. Then, under the action of pressure, the mixture is extruded onto a substrate, the layer thickness being formed in dependence on the applied pressure used. The applied sensitive layer is polymerized, polycondensed or hardened and the layer is also homogenized by swelling in a fluorescence indicator solution.
39 Citations
31 Claims
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1. Method of manufacturing a single-layer system for measuring concentration of analytes, comprising:
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adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×
104 Pa onto a substrate to obtain a sensitive layer having a thickness and shape;polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator.
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2. The method according to claim 1, wherein the applying comprises extruding the mixture into a mold to obtain the sensitive layer on a position on the substrate.
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3. The method according to claim 1, wherein the permeable material comprises polydimethylsiloxane.
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4. The method according to claim 1, wherein the solvent comprises methylene chloride.
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5. The method according to claim 1, wherein the filler material comprises member selected from the group consisting of silica gel and porous glass.
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6. The method according to claim 1, wherein the applying under pressure comprises compressing in a mold.
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7. The method according to claim 1, wherein the matrix material comprises a polysiloxane.
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8. The method according to claim 1, wherein the substrate comprises a reflective substrate.
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9. The method according to claim 1, wherein the substrate comprises a reflective substrate.
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10. The method according to claim 1, wherein the substrate comprises a member selected from the group consisting of an optical fiber, a bundle of optical fibers, a plate, a lens, a prism and an integrated optical structure.
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11. Sensitive-single layer system produced by the method according to claim 1.
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12. The method according to claim 1, wherein the applying under pressure comprises utilizing a pressure of 15×
- 104 Pa.
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13. Sensitive-single layer system produced by the method according to claim 12.
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14. The method according to claim 1, wherein the permeable material comprises a polysiloxane;
- the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10-1 to 10-6 ;
the layer includes an overall concentration of fluorescence indicator in the matrix of 10-1 to 10-6 mol/l;
the filler material comprises a member selected from the group consisting of silica gel and porous glass; and
the filler material is contained in the matrix material with a weight proportion of 5 to 65% by weight.
- the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10-1 to 10-6 ;
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15. Sensitive-single layer system produced by the method according to claim 14.
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16. The method according to claim 1, wherein the fluorescence indicator comprises a ruthenium-diamine complex.
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17. Sensitive-single layer system produced by the method according to claim 16.
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18. The method according to claim 1, wherein the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10-1 to 10-6.
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19. The method according to claim 18, wherein the solvent comprises a solution containing the fluorescence indicator in a molar concentration of 10-3 to 10-4.
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20. The method according to claim 18, wherein the solution comprises a methylene chloride solution.
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21. Sensitive-single layer system produced by the method according to claim 18.
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22. The method according to claim 1, wherein the layer includes an overall concentration of fluorescence indicator in the matrix of 10-1 to 10-6 mol/l.
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23. The method according to claim 22, wherein the layer includes an overall concentration of fluorescence indicator in the matrix of 10-2 to 10-4 mol/l.
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24. Sensitive-single layer system produced by the method according to claim 22.
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25. The method according to claim 1, wherein the filler material is contained in the matrix material with a weight proportion of 5 to 65% by weight.
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26. The method according to claim 25, wherein the filler material is contained in the matrix material with a weight proportion of 20 to 30% by weight.
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27. The method according to claim 25, wherein said filler material comprises silica gel.
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28. The method according to claim 25, wherein said filler material comprised porous glass.
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29. Method of manufacturing a single-layer system for measuring concentration of analytes, comprising:
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adsorbing fluorescence indicator on filler material forming fluorescence indicator adsorbed filler material; mixing the fluorescence indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under pressure onto a substrate to obtain a sensitive layer having a thickness and shape; polymerizing, polycondensing or hardening the matrix material; homogenizing the layer by swelling with a solvent capable of dissolving the fluorescence indicator; and wherein the substrate comprises a lens, and said lens comprises a GRIN lens.
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30. Method of manufacturing a single-layer system for measuring concentration of analytes, comprising:
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adsorbing indicator on filler material forming indicator adsorbed filler material; mixing the indicator adsorbed filler material with a permeable matrix material forming a mixture; applying the mixture under a pressure of 12 to 20×
104 Pa onto a substrate to obtain a sensitive layer having a thickness and shape;polymerizing, polycondensing or hardening the matrix material; and homogenizing the layer by swelling with a solvent capable of dissolving the indicator.
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31. Sensitive-single layer system produced by the method according to claim 30.
Specification