Enzyme amplification and purification process for producing DNA polymerase

  • US 6,017,745 A
  • Filed: 03/30/1995
  • Issued: 01/25/2000
  • Est. Priority Date: 08/07/1984
  • Status: Expired due to Term
First Claim
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1. A recombinant plasmid containing a DNA coding sequence for the expression of DNA polymerase activity, wherein said DNA coding sequence is derived from a source that encodes a bacterial DNA Polymerase, said source not containing an amber mutation affecting expression of said DNA polymerase activity, such that when said plasmid is transformed into a bacterial host system the host system can grow and divide thereby replicating said plasmid.

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