Electrochemical detector integrated on microfabricated capilliary electrophoresis chips

• US 6,045,676 A
• Filed: 08/22/1997
• Issued: 04/04/2000
• Est. Priority Date: 08/26/1996
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Lao, Kaigin, Woolley, Adam T., Mathies, Richard A., Glazer, Alexander N.
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US08/916,557
• Time in Patent Office: 956 Days
• Field of Search: 204/601-605, 204/451-455
• US Class Current: 204/603
• CPC Class Codes: G01N 27/4473 by electric means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize

Abstract

A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.

Claims

1. In a microfabricated capillary electrophoresis chip including a substrate with an elongated separation channel with conductive means at each end of the channel for applying a separation voltage along the channel, the improvement comprising an integrated electrochemical detector having at least one thin film working electrode extending into said separation channel at or very near one end of said separation channel adapted to detect molecules as they migrate past the thin film electrode after they have migrated down the channel, a portion of the thin film working electrode extending into said channel being very narrow in the direction of the separation electric field to minimize the effect of the electrophoresis voltage gradient which it senses along the channel and a reference electrode spaced 1-2000 μ

m from the thin film working electrode.

2. A microfabricated capillary electrophoresis chip as in claim 1 in which said channel is from 1-500 μ

m wide and 1-500 μ

m deep, said narrow end of the thin film working electrode is from 1-500 μ

m wide and the spacing between the ends of the thin film working electrode and reference electrode is from 1-500 μ

m.

3. A microfabricated capillary electrophoresis chip as in claim 1 in which the channel is from 1-2000 μ

m wide and 1-2000 μ

m deep, said narrow end of the thin film working electrode is from 1-2000 μ

m wide.

4. A microfabricated capillary electrophoresis chip as in claim 1 in which the channel at said one end transitions to a larger volume portion and said thin film working electrode and reference electrode are placed in or near the transition to a larger volume.

5. A microfabricated capillary electrophoresis chip as in claim 1 including a plurality of pairs of spaced thin film working electrodes disposed near said end of said channel to perform multiple potential electrochemical detection.

6. A microfabricated capillary electrophoresis chip as in claim 1 including thin film leads extending from said electrodes to the edge of said chip.

7. A microfabricated capillary electrophoresis chip as in claim 1 in which the channel transitions to a larger volume and said thin film working electrode is in said larger volume within 500 μ

m of the transition.

8. A microfabricated capillary electrophoresis chip as in claim 1 in which the channel transitions to a larger volume and said thin film working electrode is in said larger volume within 2000 μ

m of the transition.

9. A microfabricated capillary electrophoresis chip as in claim 1 including a plurality of spaced thin film working electrodes disposed near said end of said channel to perform multiple potential electrochemical detection.