Process and device for generating resonance phenomena in particle suspensions

• US 6,056,861 A
• Filed: 07/27/1998
• Issued: 05/02/2000
• Est. Priority Date: 11/27/1995
• Status: Expired due to Term

• Current Assignee: Gunter Fuhr
• Original Assignee: Gunter Fuhr
• Inventors: Gimsa, Jan, Schnelle, Thomas, Muller, Torsten, Fuhr, Gunter
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/077,334
• Time in Patent Office: 645 Days
• Field of Search: 204/547, 204/643, 204/450, 204/458, 204/600, 204/609, 204/400, 205/775
• US Class Current: 204/547
• CPC Class Codes: B03C 5/028 using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
• CPC Class Codes: C12M 33/00 Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: H02N 13/00 Clutches or holding devices using electrostatic attraction, e.g. using Johnson-Rahbek effect

Abstract

A method and a device for position and/or type-selective control of the position and/or change of position of suspended particles in a multielectrode system by the effect of polarization forces that are induced in the particles by alternating electric fields in the multielectrode system, which particles comprise biological or synthetic objects with dimensions essentially corresponding to those of biological cells or cell organelles, viruses or macromolecules, base on the fact that the multielectrode system forms with the particle suspension an electrical network, in which means of resonance are provided for creating a resonant increase or damping of the field strength of the alternating electric fields at certain frequencies in at least one locally demarcated region of the multielectrode system.

Claims

1. Method for position and/or type-selective control of the position and/or change of position of suspended particles in a multielectrode system by the effect of polarization forces that are induced in the particles by alternating electric fields in the multielectrode system, which particles comprise biological or synthetic objects with dimensions essentially corresponding to those of biological cells or cell organelles, viruses or macromolecules, characterized in that the polarization forces are amplified or reduced by increased resonance or damping of the field strength of the alternating electric fields at certain frequencies in at least one locally demarcated region of the multielectrode system, the alternating electric fields including alternating, rotating or traveling electric fields so that, in circular and/or linear multielectrode configurations, there is rotation, translation or positioning of particles induced by the principle of electrorotation, dielectrophoresis, levitation or traveling-wave technology, whereby certain frequency ranges of the particular motion are amplified or damped by the resonance phenomena.

2. Method according to claim 1 in which the resonant change of field strength is achieved by the external adjustment of controllable components provided in the multielectrode system.

3. Method according to claim 1 in which the resonant increase of field strength is influenced selectively by the passive electrical characteristics of the particle suspension, whereby, especially through the presence or passage of one or more particles in a region in the multielectrode system, the electrical characteristics of the suspension at this point are altered so that the resonant response of the microstructure is altered, defined or electronically tuned so that the resonance conditions, possibly selective in time through alteration of the passive electrical characteristics of the suspension, are only achieved or terminated until the presence or passage of a certain particle type by this particle itself.

4. Method according to claim 1 in which the resonant increase of field strength is achieved at a certain fundamental of the alternating electric fields and/or multiples of the fundamental with amplification by a factor of approx. two to 1000, whereby in particular the alternating electric fields are generated by periodic control voltages of a frequency ≧

100 Hz with amplitudes between 0.1 and 200 V and the periodic signals applied for field generation can be sinusoidal, triangular, squarewave, tristate or combinations of these signals, of which certain Fourier components are possibly amplified by the resonance, which Fourier components of the periodic control signal can simultaneously generate field components of different rotation or translation sense, whereby the amplitudes of the Fourier components of the field can be attuned to one another by the resonance.

5. Method according to claim 1 in which holding of the particles is amplified or damped by the resonance phenomena.

6. Method according to claim 1 in which the particles comprise a mixture of different types of particles and the resonances alter the motion of part or all of these particle types, whereby one or more particle types alter from negative to positive dielectrophoresis at the resonant frequency or the sense of particle rotation or motion in the traveling-wave field.

7. Method according to claim 1 in which two or more field frequencies are modulated or used simultaneously by different electrode subsystems or used alternating with the same or opposite sense of rotation or translation and adjustable amplitude in that the resonant frequency is applied to ranges of the dielectric particle spectrum (force as function of frequency) where the particle types differ.

8. Device for position and/or type-selective control of the position and/or change of position of suspended particles in a multielectrode system by the effect of polarization forces that are induced in the particles by alternating electric fields in the multielectrode system, which particles comprise biological or synthetic objects with dimensions essentially corresponding to those of biological cells or cell organelles, viruses or macromolecules, characterized in that the multielectrode system forms with the particle suspension an electrical network, in which resonance means are provided for creating a resonant increase or damping of the field strength of the alternating electric fields at certain frequencies in at least one locally demarcated region of the multielectrode system, resonance means being formed by the capacitive and/or inductive design of the electrodes of the microelectrode system.

9. Control device according to claim 8 in which means the resonance are formed by controllable components integrated into or added to the multielectrode system.

10. Control device according to claim 8 in which the resonance means are formed by the particle suspension, especially the particles themselves as components of the electrical network.

11. Control device according to claim 8 in which the microelectrode configurations exhibit typical gap dimensions from 10 nm to several hundred μ

m and in which rotating or alternating electric fields are generated.

12. Control device according to claim 8 in which the microelectrode configurations have three-dimensional structures or multilayer structures on a substrate consisting of glass, semiconductor material, plastic or ceramic.

13. Control device according to claim 8 in which the substrate has structures, passive components, areas with channels, walls, trenches, cutouts or barriers, and/or micromechanical elements like valves, membranes, shiftable elements or moving arms for tuning the oscillating circuits, or in which the output stages or the entire high-frequency generator for producing the electrode signals and/or driving the components to control the resonant frequencies are integrated on the substrate.

14. Control device according to claim 8 in which several microelectrode systems are configured next to one another, back to back, offset, facing one another, as a cascade, in a ring or stacked.

15. Control device according to claim 8 in which components are provided with which the resonant frequencies are controlled and which can be operated by a control program, which components are formed of active components like variable-capacitance diodes, field-effect transistors and adjustable inductors, whereby the alterations in the resonance phenomena can also be generated by connecting, disconnecting or bypassing components of the oscillating circuits.

16. Control device according to claim 8, in which the electrodes on essentially planar, insulating mounts form at least an open electrode system that has capacitive, inductive and resistive components and is connected through the particle suspension solution to at least one network-type oscillating circuit system.

17. Control device according to claim 16, in which the electrode system takes the form of meanders and/or loops covered by the insulating layers, which form networks with altered influence of capacitive and/or inductive elements, whereby separate resonance regions influence one another.

18. Method for position and/or type-selective control of the position and/or change of position of suspended particles in a multielectrode system by the effect of polarization forces that are induced in the particles by alternating electric fields in the multielectrode system, which particles comprise biological or synthetic objects with dimensions essentially corresponding to those of biological cells or cell organelles, viruses or macromolecules, characterized in that the polarization forces are amplified or reduced by increased resonance or damping of the field strength of the alternating electric fields at certain frequencies in at least one locally demarcated region of the multielectrode system, two or more field frequencies being modulated or used alternating with the same or opposite sense of rotation or translation and adjustable amplitude in that a resonant frequency is applied to ranges of the dielectric particle spectrum of said particles where the particle types differ.

19. Device for position and/or type-selective control of the position and/or change of position of suspended particles in a multielectrode system by the effect of polarization forces that are induced in the particles by alternating electric fields in the multielectrode system, which particles comprise biological or synthetic objects with dimensions essentially corresponding to those of biological cells or cell organelles, viruses or macromolecules, characterized in that the multielectrode system forms with the particle suspension an electrical network, in which resonance means are provided for creating a resonant increase or damping of the field strength of the alternating electric fields at certain frequencies in at least one locally demarcated region of the multielectrode system, the electrodes on essentially planar, insulating mounts forming at least an open electrode system that has capacitive, inductive and resistive components connected through the particle suspension solution to at least one network-type oscillating circuit system, the electrode system being in the form of meanders and/or loops covered by the insulating layers, which form a network with altered influence of capacitive and/or inductive elements, whereby separate resonance regions influence one another.