Disease diagnosis by vapor sample analysis

• US 6,057,162 A
• Filed: 09/12/1997
• Issued: 05/02/2000
• Est. Priority Date: 03/07/1997
• Status: Expired due to Fees

• Current Assignee: Thermedics Detection, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Thermedics Detection, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Rounbehler, David P., Wheeler, David B., Jarvis, George B., Fine, David H.
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/928,735
• Time in Patent Office: 963 Days
• Field of Search: 436/119, 436/120, 436/121, 436/122, 436/135, 436/139, 436/172, 436/900, 422/52, 422/82.05, 422/82.08, 422/83, 422/84, 422/89, 422/98, 73/23.2, 73/23.34, 600/532
• US Class Current: 436/119
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: Y10T 436/18 Sulfur containing
• CPC Class Codes: Y10T 436/182 Organic or sulfhydryl containing [e.g., mercaptan, hydrogen, sulfide, etc.]
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide

Abstract

Diagnosing disease in a body by analyzing a sample from the body for the presence of sulfur compounds.

Claims

1. A method of assisting diagnoses of disease in biological matter, comprising:

obtaining a vapor sample from said biological matter, analyzing the vapor sample by luminescent reaction with ozone for the presence of sulfur compounds in a concentration range of about 500 femtograms or less, and assisting diagnoses of disease based on said analyzing.

2. The method of claim 1, comprising processing said sample by gas chromatography prior to said analyzing.

3. The method of claim 2 comprising reporting the presence or absence of disease based on a profile of particular sulfur compounds.

4. The method of claim 3 comprising assisting diagnoses of the presence or absence of disease based on the profile of dimethyl disulfide, methyl mercaptan, hydrogen sulfide, dimethyl sulfide or sulfur dioxide.

5. The method of claim 1 further comprising detecting said sulfur compounds in a concentration range of about 25 femtograms or less.

6. The method of claim 1 comprising quantifying said sulfur compounds.

7. The method of claim 1, wherein said vapor sample is exposed to a pressure between about 0.5 atm to about 3 atm after obtaining said sample.

18. The method of claims 1, 3, 5, or 8, comprising treating said vapor sample prior to luminescent reaction with ozone by converting sulfur compounds to reactive species without complete reduction to H₂ S or SO.

19. The method of claim 18 wherein said conversion of said sulfur compounds is by exposure to a heated metal, without added reducing agent.

20. The method of claim 19 wherein the metal is stainless steel or nickel.

8. A method of detecting a sulfur compound from biological matter, comprising:

obtaining a vapor sample containing a sulfur compound from biological matter,

treating said vapor sample by exposing the sulfur compound in said vapor sample to a heated metal, without added reducing agent,

exposing said treated sample to ozone,

detecting luminescence generated by exposing said treated sample to ozone, and

analyzing said luminescence to determine the presence of said sulfur compound.

9. The method of claim 8 wherein said metal is heated to about 350°

C.

10. The method of claim 8 or 9 wherein said metal comprises nickel or stainless steel.

11. The method of claim 8 or 9 comprising pretreating said vapor sample by separating compounds in said sample.

12. The method of claim 11 comprising pretreating said vapor sample, by gas chromatography.

13. The method of claim 8 or 9 comprising obtaining said vapor sample by collecting vapor from an animal body.

14. The method of claim 13 comprising heating the body to aid release of vapor.

15. The method of claim 8 or 9 wherein said sulfur compound is selected from the group consisting of methyl mercaptan, dimethyl sulfide and dimethyl disulfide.

16. The method of claim 8 or 9 comprising analyzing said luminescence to determine the amount of sulfur compound in said vapor sample.

17. The method of claim 8 or 9 comprising exposing said vapor sample to a heated metal in the presence of air.