Molecular wire injection sensors

• US 6,060,327 A
• Filed: 05/14/1997
• Issued: 05/09/2000
• Est. Priority Date: 05/14/1997
• Status: Expired due to Fees

• Current Assignee: Keensense, Inc.
• Original Assignee: Keensense, Inc.
• Inventors: Keen, Randy E.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/856,822
• Time in Patent Office: 1,091 Days
• Field of Search: 204/400, 204/403, 422/82.01, 422/82.02, 435/6, 435/287.1, 435/287.2, 436/518, 436/524, 436/525, 436/531, 436/149, 436/150, 436/151, 436/806
• US Class Current: 506/9
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Disclosed is a sensor for sensing the presence of an analyte component without relying on redox mediators. This sensor includes (a) a plurality of conductive polymer strands each having at least a first end and a second end and each aligned in a substantially common orientation; (b) a plurality of molecular recognition headgroups having an affinity for the analyte component and being attached to the first ends of the conductive polymer strands; and (c) an electrode substrate attached to the conductive polymer strands at the second ends. The electrode substrate is capable of reporting to an electronic circuit reception of mobile charge carriers (electrons or holes) from the conductive polymer strands. The electrode substrate may be a photovoltaic diode.

Also disclosed is method of forming a sensor capable of sensing the presence of an analyte component. This method includes (a) contacting a sensor substrate (e.g., a device element of a device on semiconductor chip) with a first medium containing mobile conductive polymer strands or precursors of the conductive polymer strands; (b) applying a first potential to the substrate sufficient to form a first structure having the conductive polymer strands affixed to the substrate; (c) contacting the sensor substrate, with affixed conductive polymer strands, with a second medium containing mobile molecular recognition headgroups; and (d) applying a second potential to the substrate sufficient to affix the molecular recognition headgroups to the affixed conductive polymer strands.

Claims

1. A sensor for sensing the presence of an analyte component, which sensor does not rely on redox mediators, the sensor comprising:

a plurality of conductive polymer strands each having at least a first end and a second end and each aligned in a non random orientation;

a plurality of molecular recognition headgroups having an affinity for said analyte component and participating in a redox reaction when contacting a molecule of said analyte component, said plurality of headgroups being attached to said conductive polymer strands such that when said redox reaction occurs at a headgroup, a mobile charge carrier is transferred directly to a conductive polymer strand attached to said headgroup, without redox reaction in the polymer strand; and

an electrode substrate attached to said conductive polymer strands at said second ends that reports to an electronic circuit reception of mobile charge carriers from said conductive polymer strands web the presence of said analyte component is sensed.

2. The sensor of claim 1, wherein the plurality of conductive polymer strands are multi-stranded nucleic acid strands.

3. The sensor of claim 2, wherein the plurality of conductive polymer strands are double-stranded DNA strands.

4. The sensor of claim 1, wherein the plurality of conductive polymer strands are selected from the group consisting of multi-stranded nucleic acids, electron transport proteins, biopolymers, synthetic organic and inorganic conducting polymers, metal crystallite molecular wires, and Langmuir-Blodgett conducting films.

5. The sensor of claim 1, wherein the plurality of conductive polymer strands have an orientation that is preferentially orthogonal to the electrode substrate.

6. The sensor of claim 1, wherein the plurality of molecular recognition headgroups participate in said redox reaction by catalyzing a chemical transformation of said analyte component.

7. The sensor of claim 1, wherein the plurality of molecular recognition headgroups are selected from the group consisting of oxidoreductases and catalytic antibodies.

8. The sensor of claim 1, wherein the plurality of molecular recognition headgroups are chemically homogeneous.

9. The sensor of claim 1, wherein the plurality of molecular recognition headgroups are chemically inhomogeneous.

10. The sensor of claim 9, wherein the sensor includes a first region on said electrode substrate where a first group of chemically homogeneous molecular recognition headgroups is located and second region on said electrode substrate where a second group of chemically homogeneous molecular recognition headgroups is located, and wherein the first and second regions are separately addressable.

11. The sensor of claim 1, wherein the molecular recognition headgroups include glucose oxidase.

12. The sensor of claim 1, wherein the electrode substrate is one electrode of a photovoltaic diode.

13. The sensor of claim 1, wherein the electrode substrate is a component of a semiconductor chip.

14. The sensor of claim 1, wherein the conductive polymer strands are superconducting.

15. A sensor for sensing the presence of an analyte component, the sensor comprising:

a plurality of multi-stranded nucleic acid wires each having at least a first end and a second end;

a plurality of molecular recognition headgroups having an affinity for said analyte component and participating in a redox reaction when contacting a molecule of said analyte component, said plurality of headgroups being attached to said multi-stranded nucleic acid strands; and

an electrode substrate attached to said multi-stranded nucleic acid strands at said second ends that reports to an electronic circuit reception of mobile charge carriers from said multi-stranded nucleic acid strands whereby the presence of said analyte component is sensed.

16. The sensor of claim 15, wherein the plurality of multi-stranded nucleic acid strands are attached to said molecular recognition headgroups such that mobile charge carriers are transferred directly to said multi-stranded nucleic acid strands when said redox reaction occurs, without the need for a mediator.

17. The sensor of claim 15, wherein said plurality of multi-stranded nucleic acid strands are each aligned in a non random orientation.

18. The sensor of claim 15, wherein the plurality of multi-stranded nucleic acid wires are double-stranded DNA wires.

19. The sensor of claim 15, wherein the plurality of multi-stranded nucleic acid wires are double-stranded DNA wires having a B-DNA conformation.

20. The sensor of claim 15, wherein the electrode substrate is a component of a semiconductor chip.

21. A sensor for sensing the presence of an analyte component, which sensor does not rely on redox mediators, the sensor comprising:

a plurality of molecular recognition headgroups having an affinity for said analyte component and participating in a redox reaction when contacting a molecule of said analyte component such that when said redox reaction occurs at a headgroup, a mobile charge carrier is generated;

a diode having a first electrode to which said plurality of molecular recognition headgroups are affixed such that mobile charge carriers generated by said redox reaction are transferred to said first electrode; and

a circuit for detecting when said mobile charge carriers are transferred to said first electrode whereby the presence of said analyte component is sensed.

22. The sensor of claim 21, wherein the plurality of molecular recognition headgroups are attached to a p-type side of said diode.

23. The sensor of claim 21, wherein the plurality of molecular recognition headgroups are attached to the first electrode via a plurality of conductive polymer strands selected from the group consisting of multi-stranded nucleic acids, electron transport proteins, biopolymers, synthetic organic and inorganic conducting polymers, metal crystallite molecular wires, and Langmuir-Blodgett conducting films.

24. The sensor of claim 23, wherein the plurality of conductive polymer strands are non randomly oriented.

25. The sensor of claim 21, wherein the plurality of molecular recognition headgroups are selected from the group consisting of oxidoreductases and catalytic antibodies.

26. The sensor of claim 21, wherein the plurality of molecular recognition headgroups are chemically inhomogeneous.

27. The sensor of claim 26, wherein the sensor includes a first region on said electrode substrate where a first group of chemically homogeneous molecular recognition headgroups is located and second region on said electrode substrate where a second group of chemically homogeneous molecular recognition headgroups is located, and wherein the first and second regions are separately addressable.

28. The sensor of claim 21, wherein the diode is a device on semiconductor chip.

29. The sensor of claim 21, wherein the diode is a photovoltaic diode.

30. A sensor for sensing the presence of a nucleic acid sequence, the sensor comprising:

a plurality of sequence-specific single-stranded nonconductive nucleic acid wires each having at least a first end and a second end;

an electrode substrate attached to said sequence-specific single-stranded nonconductive nucleic acid strands at said second ends that reports to an electronic circuit, reception of mobile charge carriers originating from complementary multi-stranded nucleic acid strands;

molecular recognition headgroups arranged to inject mobile charge carriers into nucleic acid wires whereby when said sensor is exposed to an analyte having said complementary nucleic acid sequence, at least some of said single-stranded nonconductive nucleic acid wires hybridize with said analyte to form conductive multi-stranded nucleic acid strands whereby the presence of said nucleic acid sequence is sensed.

31. The sensor of claim 30, wherein the plurality of sequence-specific single-stranded nonconductive nucleic acid strands are attached to the molecular recognition headgroups at said first ends, such that mobile charge carriers are transferred to said multi-stranded nucleic acid strands when a redox reaction occurs at said molecular recognition headgroups, wherein said multi-stranded nucleic acid strands are formed by hybridization between said sequence-specific single-stranded nonconductive nucleic acid strands and said analyte having said complementary nucleic acid sequence.

32. The sensor of claim 30, wherein the electrode substrate is a is a component of a semiconductor chip.

33. A sensor for sensing the presence of an analyte component, which sensor does not rely on redox mediators, the sensor comprising.

a plurality of conductive polymer strands each having at least a first end and a second end;

a plurality of molecular recognition headgroups having an affinity for said analyte component and participating in a redox reaction when contacting a molecule of said analyte component, said plurality of headgroups being attached to said first ends of said conductive polymer strands such that when said redox reaction occurs at a headgroup, a mobile charge carrier is transferred directly to a conductive polymer strand attached to said headgroup, without redox reaction in the polymer strand; and

an electrode substrate attached to said conductive polymer strands at said second ends that reports to an electronic circuit reception of mobile charge card ers from said conductive polymer strands whereby the presence of said analyte component is sensed, wherein the conductive polymer strands'"'"' conductivity reversibly changes between conductive and insulative states.

34. The sensor of claim 33, wherein the molecular recognition headgroup is selected from the group consisting of esterases, amidases, acylases, and lipases.

35. The sensor of claim 33, wherein the plurality of conductive polymer strands includes a polymer polymerized from monomers selected from the group consisting of N-methylpyrrole, aniline, thiophene, 3-methylthiophene, 3,4-dimethylthiophene, vinylferrocene, styrene, nitrostyrene, viologens, vinyl-pyridine, vinyl-2,2'"'"'-bipyridine, vinylrubrene, quinone-based compounds, and derivatives thereof.

36. The sensor of claim 33, wherein the conductivity of the conductive polymer changes in response to changes in pH.