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Non-contract method for assay reagent volume dispense verification

  • US 6,097,831 A
  • Filed: 10/14/1997
  • Issued: 08/01/2000
  • Est. Priority Date: 10/14/1997
  • Status: Expired due to Fees
First Claim
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1. A method for detecting anti-ligand in a fluid dispensed into a reaction chamber of an automated affinity assay vessel having a volume determination reference point, said reaction chamber having a test strip disposed therein, said test strip having a immobilized ligand capable of complexing said anti-ligand, said method comprising the steps of:

  • a) dispensing the fluid into said reaction chamber;

    b) agitating said fluid in said reaction chamber to promote contact between said fluid and said test strip;

    c) without contacting said fluid, determining the relative positions of an edge of the meniscus of said fluid and said volume determination reference point;

    d) calculating the volume of said fluid contained in said vessel from said relative positions within an error tolerance of less than 10%; and

    e) automatically remotely inspecting the test strip in situ by a method comprising;

    (i) illuminating the test strip, in said chamber with light from a light emission source;

    (ii) reading the light reflected from the test strip with a video imaging system; and

    (iii) analyzing the reflected light to determine whether the immobilized ligand on the test strip has complexed the anti-ligand.

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