Blood cell count/immunoassay apparatus using whole blood

• US 6,106,778 A
• Filed: 09/25/1998
• Issued: 08/22/2000
• Est. Priority Date: 09/27/1997
• Status: Expired due to Term

• Current Assignee: Horiba Limited
• Original Assignee: Horiba Limited
• Inventors: Oku, Narihiro, Yamao, Yasuo
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): Do, Pensee T.
• Application Number:
  US09/161,127
• Time in Patent Office: 697 Days
• Field of Search: 422/50, 422/55, 422/58, 422/63, 422/67, 422/68.1, 422/73, 422/82.05, 422/81, 422/99, 422/102, 422/103, 422/105, 422/108, 422/119, 422/187, 422/188, 422/189, 422/190, 422/192 , 422/193, 422/224, 422/225, 422/236, 436/43, 436/63, 436/164, 436/174, 436/179, 436/175, 436/180, 436/518, 436/522, 436/523, 436/805
• US Class Current: 422/50
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/10 Investigating individual particles
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A compact combination blood cell count and immunoassay apparatus for measuring drawn whole blood specimen includes a load station for receiving the whole blood specimen and a nozzle member that can selectively withdraw whole blood from the blood specimen. A blood cell count section and an immunoassay section are mounted along a unitary path of movement of the nozzle member so that a single nozzle member may be used for transporting the whole blood to the respective blood cell count section and immunoassay section. A controller can automatically direct a transport mechanism to use only the whole blood and to perform various steps of adding dilution liquid and reagents, along with appropriate washing of the nozzle and sample cells to insure an automatic operation. A hematozorit value can be determined from the blood cell count signal and used to correct the immunoassay signal when whole blood is used.

Claims

1. A combination blood cell count and an immunoassay apparatus can measure a drawn whole blood specimen positioned at a load station, comprising

a nozzle member for releaseably holding a portion of the whole blood withdrawn from the load station;

means for adding a dilution liquid;

means for adding reagents;

a blood cell count section for providing signals on blood cells;

an immunoassay section for providing signals on immunoassay;

a transport mechanism for moving the nozzle member to the blood cell count section and the immunoassay section;

a controller for directing the transport mechanism to transport the nozzle member to the blood cell count section and immunoassay section and to mix reagents with the whole blood for the immunoassay section and to dilute the whole blood with the dilution liquid for the blood cell count section; and

means for determining a hematocrit from a blood cell count signal and correcting the immunoassay signal with the hematocrit.

2. The apparatus of claim 1, wherein the blood cell count is performed in the blood cell count section, while immunoreactions are taking place in the immunoassay section.

3. The apparatus of claim 1, wherein the load station, immunoassay section and blood cell count section are arranged in a straight line beneath the nozzle member.

4. The apparatus of claim 1, wherein a cell in the blood cell count section is also used as a waste liquid chamber for washing the nozzle member.

5. A combination blood cell count and an immunoassay apparatus comprising:

a nozzle member for holding a sample of whole blood;

a blood cell count section for receiving said sample of whole blood from the nozzle member and providing a hematocrit signal;

an immunoassay section for receiving whole blood from the nozzle member and providing an immunoassay signal; and

correcting means for correcting the immunoassay signal with the hematocrit signal.

6. The apparatus of claim 5, wherein the blood cell count is performed in the blood cell count section, while immunoreactions are taking place in the immunoassay section.

7. The apparatus of claim 5, wherein the load station, the immunoassay section and the blood cell count section are arranged in a straight line beneath the nozzle member.

8. The apparatus of claim 5, wherein a cell in the blood cell count section is also used as a waste liquid chamber for washing the nozzle member.

9. A compact combination blood cell count and an immunoassay apparatus for measuring drawn whole blood specimen, comprising:

a load station for receiving a whole blood specimen;

a blood cell count section for providing signals on blood cells;

an immunoassay section for providing signals on immunoassay;

a nozzle member for releaseably holding a portion of the whole blood withdrawn from the load station and delivering the whole blood to each of the blood cell count section and the immunoassay section;

a transport mechanism for moving the nozzle member to the blood cell count section and immunoassay section, the transport mechanism providing a unitary path of movement for the nozzle member to reciprocate along in a horizontal direction above the load station, blood cell count section and immunoassay section and to vertically move the nozzle member for operative contact with the load station, blood cell count section and immunoassay section;

a controller for automatically directing the transport mechanism to transport the nozzle member to the blood cell count section and the immunoassay section; and

means for determining a hematocrit from a blood cell count signal and correcting the immunoassay signal with the hematocrit.

10. The apparatus of claim 9, wherein the load station, the immunoassay section and the blood cell count section are arranged in a straight line beneath the nozzle member.

11. The apparatus of claim 9, wherein a cell in the blood cell count section is also used as a waste liquid chamber for washing the nozzle member.

12. The apparatus of claim 9, wherein the blood cell count is performed in the blood cell count section, while immunoreactions are taking place in the immunoassay section.

13. A combination blood cell count and an immunoassay apparatus for automatically measuring the properties of a whole blood specimen, comprising:

a load station for receiving a whole blood specimen drawn directly from a patient;

a single nozzle member for releaseably holding a portion of the whole blood withdrawn from the blood specimen located at the load station;

means for adding dilution liquid;

means for adding reagents;

a blood cell count section for providing signals on blood cells;

an immunoassay section for providing signals on immunoassay;

a transport mechanism for moving the nozzle member to the blood cell count section and the immunoassay section;

means for repetitively washing the nozzle member along its travels from the load station to respectively the blood cell count section and the immunoassay section;

a controller for automatically directing the transport mechanism to transport the single nozzle member to the blood cell count section and immunoassay section and to mix reagents with the whole blood for the immunoassay section and to dilute the whole blood with a dilution liquid for the blood cell count section and to wash the single nozzle member at predetermined positions during its travel; and

means for determining a hematocrit from a blood cell count signal and correcting the immunoassay signal with the hematocrit.

14. The apparatus of claim 13, wherein the blood cell count is performed in the blood cell count section, while immunoreactions are taking place in the immunoassay section.

15. The apparatus of claim 14, wherein the load station, immunoassay section and blood cell count section are arranged in a straight line beneath the nozzle member.

16. The apparatus of claim 15, wherein a cell in the blood cell count section is also used as a waste liquid chamber for washing the nozzle member.