Reagent for measurement of the haemoglobin and determination of the leukocytes in a blood sample

• US 6,114,130 A
• Filed: 07/09/1999
• Issued: 09/05/2000
• Est. Priority Date: 08/04/1998
• Status: Expired due to Term

• Current Assignee: ABX
• Original Assignee: ABX
• Inventors: Champseix, Henri, Veriac, Sylvie
• Primary Examiner(s): Leary, Louise N.
• Application Number:
  US09/352,666
• Time in Patent Office: 424 Days
• Field of Search: 435/7.24, 435/4, 436/66
• US Class Current: 435/7.24
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/721 Haemoglobin

Abstract

A reagent for measurement of the haemoglobin and determination of the leukocytes in a blood sample is provided. This reagent comprises at least one detergent of the cationic type; a compound of the glycoside type, and in particular a saponin; at least one inorganic salt and/or an osmotic and/or leukoprotective agent; and an organic and/or inorganic buffer which can adjust the pH of the agent selectively, either to a substantially neutral value, e.g. pH between 5 and 8, or to a basic value, e.g. pH between 8 and 12. The reagent can be used in haematological analyses in human and veterinary medicine.

Claims

1. A reagent for measurement of the haemoglobin and determination of the leukocytes in a blood sample, which comprises:

at least one detergent of the cationic type;

a compound of the glycoside type;

at least one inorganic salt and/or an osmotic and/or leuko-protective agent; and

an organic and/or inorganic buffer, which can adjust selectively the pH of the reagent, either to a substantially neutral value of between 5 and 8, or to a basic value of between 8 and 12.

2. The reagent of claim 1, wherein the detergent is selected from the group consisting of:

the primary amines, acetates and hydrochlorates of fatty amines;

the salts of quaternary ammonium, and the bromide of trimethylketyl ammonium;

the amides of substituted diamines, which are rendered cationic by ethyl sulphate, diethanolamino-propylamine, diethylamino-propylamide; and

the amides of ring-formed diethylenetriamine.

3. The reagent of claim 2, wherein the detergent is present in a concentration of between 0 and 50 g/l.

4. The reagent of claim 1, wherein the compound of the glycoside type is selected from the group consisting of saponins and saponosides.

5. The reagent of claim 1, wherein the glycoside compound is present in a concentration of between 0.5 and 20 g/l.

6. The reagent of claim 1, wherein the inorganic salt is selected from the group consisting of chloride, sulphate or fluoride of sodium and potassium.

7. The reagent of claim 1, wherein the inorganic salt is present in a concentration of between 1 and 15 g/l.

8. The reagent of claim 1, wherein the osmotic and/or leuko-protective agent is selected from the group consisting of mannitol and D-glucose.

9. The reagent of claim 1, wherein the osmotic and/or leuko-protective agent is present in a concentration of between 0 and 30 g/l.

10. The reagent of claim 1, wherein the organic and/or inorganic buffer is selected from the group consisting of:

triethanolamine;

hydrogenated phosphates of sodium and potassium;

N-[2-acetamido]-2 iminodiacetic acid;

N-[carbamoylmethyl] iminodiacetic acid;

2 amino-2-methyl 1,3 propanediol;

glycine;

sodium carbonate;

citric acid; and

tris (hydroxymethyl) aminomethane.

11. The reagent of claim 1, wherein the organic and/or inorganic buffer is present in a concentration of between 0 and 2 wt. %.

12. The reagent of claim 1, wherein the buffer adjusts the pH of the reagent to a value of between 5 and 8.

13. The reagent of claim 1, wherein the buffer adjusts the pH of the reagent to a value of between 8 and 12.

14. The reagent of claim 1 being free of cyanide compounds.