Method for multiple analyte detection
First Claim
1. A method for detecting or quantitating one or more of a plurality of polynucleotide sequences in a liquid sample, said method comprisingproviding a device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain a different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal, wherein the interior of the network is under vacuum,applying a liquid sample to the sample inlet and allowing the sample to be drawn into the network by vacuum action to deliver the sample to the detection chambers,allowing the delivered sample to react with at least one sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when a specific sequence is present in the sample, andmeasuring the signals produced in the reaction chambers to detect or quantitate specific target sequences in the sample.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
193 Citations
18 Claims
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1. A method for detecting or quantitating one or more of a plurality of polynucleotide sequences in a liquid sample, said method comprising
providing a device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain a different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal, wherein the interior of the network is under vacuum, applying a liquid sample to the sample inlet and allowing the sample to be drawn into the network by vacuum action to deliver the sample to the detection chambers, allowing the delivered sample to react with at least one sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when a specific sequence is present in the sample, and measuring the signals produced in the reaction chambers to detect or quantitate specific target sequences in the sample.
Specification