Nucleic acid catalysts with endonuclease activity
First Claim
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1. A nucleic acid molecule with an endonuclease activity having the formula I:
- ##STR2## wherein, each N represents independently a nucleotide or an abasic moiety, which may be same or different;
X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
m and n are integers greater than or equal to 1, wherein if (N)m and (N)n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
J is an oligonucleotide having a sequence selected from the group consisting of 5'"'"'-GGCAUCC-3'"'"', 5'"'"'-AGCAUU-3'"'"', 5'"'"'-GCAUCA-3'"'"', 5'"'"'-AGCAUC-3'"'"', and, 5'"'"'-AGCGUC-3'"'"';
L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
represents a chemical linkage; and
C and G represent cytidine and guanosine nucleotides, respectively.
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Abstract
Nucleic acid molecules with new motifs having catalytic activity, methods of synthesis, and use thereof are also described.
193 Citations
28 Claims
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1. A nucleic acid molecule with an endonuclease activity having the formula I:
- ##STR2## wherein, each N represents independently a nucleotide or an abasic moiety, which may be same or different;
X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
m and n are integers greater than or equal to 1, wherein if (N)m and (N)n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
J is an oligonucleotide having a sequence selected from the group consisting of 5'"'"'-GGCAUCC-3'"'"', 5'"'"'-AGCAUU-3'"'"', 5'"'"'-GCAUCA-3'"'"', 5'"'"'-AGCAUC-3'"'"', and, 5'"'"'-AGCGUC-3'"'"';
L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
represents a chemical linkage; and
C and G represent cytidine and guanosine nucleotides, respectively. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
- ##STR2## wherein, each N represents independently a nucleotide or an abasic moiety, which may be same or different;
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2. A nucleic acid molecule with an endonuclease activity having the formula II:
- ##STR3## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
m and n are integers greater than or equal to 1, wherein if (N)m and (N)n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
J is an oligonucleotide of length 6 nucleotides having a sequence 5'"'"'-GGAAUC-3'"'"';
L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
represents a chemical linkage; and
C represents cytidine nucleotide.
- ##STR3## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
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3. A nucleic acid molecule with endonuclease activity having the formula III:
- ##STR4## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
X and Y are independent oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule;
m and n are integers greater than or equal to 1, wherein if (N)m and (N)n are nucleotides, (N)m and (N)n may interact by hydrogen bond interaction;
K is an oligonucleotide of length 5 nucleotides having a sequence selected from the group consisting of 5'"'"'-UGAUG-3'"'"', 5'"'"'-UGAUC-3'"'"', and 5'"'"'-UGGGC-3'"'"';
L is a linker which may be present or absent, wherein said linker, when present, is a nucleotide linker or a non-nucleotide linker, or a combination of nucleotide and a non-nucleotide linker, wherein said nucleotide linker comprises a single-stranded region, or double-stranded region, or a combination of single-stranded and a double-stranded region;
represents a chemical linkage; and
C and G represent cytidine and guanosine nucleotides, respectively.
- ##STR4## wherein, each N represents independently a nucleotide or a non-nucleotide linker, which may be same or different;
Specification