Targeted system for removing tumor cells from cell populations
First Claim
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1. A method for eliminating a first population of cells from a second population of cells, comprising:
- a) labeling said first population of cells so that said first population of cells can be distinguished from said second population of cells;
b) placing said first population of cells and said second population of cells is in a stationary position on a surface;
c) locating one cell in said first population of cells by reference to said label;
d) applying a first pulse from a controlled energy source to said one cell;
e) determining if said one cell was killed by said first pulse; and
f) applying a second pulse from a controlled energy source if said one cell was not killed.
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Abstract
A method is presented to remove contaminating tumor cells from a cell population. The method includes labeling individual tumor cells in the population and then killing them with a high energy laser beam. The laser is focused so that it specifically kills the identified tumor cell, but not the remaining cells in the population.
99 Citations
32 Claims
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1. A method for eliminating a first population of cells from a second population of cells, comprising:
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a) labeling said first population of cells so that said first population of cells can be distinguished from said second population of cells; b) placing said first population of cells and said second population of cells is in a stationary position on a surface; c) locating one cell in said first population of cells by reference to said label; d) applying a first pulse from a controlled energy source to said one cell; e) determining if said one cell was killed by said first pulse; and f) applying a second pulse from a controlled energy source if said one cell was not killed. - View Dependent Claims (2, 3, 4, 5)
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6. A method of eliminating tumor cells from within a cell population that includes non-tumor cells, comprising the steps of:
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a) labeling said non-tumor cells so that the non-tumor cells can be distinguished from said tumor cells; b) locating one of said tumor cells by identifying a non-labeled cell in said cell population; and c) killing said tumor cell by applying a pulse from a controlled energy source to said located tumor cell while said tumor cell is in a substantially stationary position on a surface. - View Dependent Claims (7, 8, 9)
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10. A method of killing a first population of cells within a mixture of cells, said mixture comprising said first population of cells and a second population of cells, comprising:
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a) illuminating a portion of said mixture of cells, wherein said first population of cells or said second population of cells is labeled such that they are substantially distinguishable from one another; b) capturing an image of a plurality of cells in the illuminated portion of said mixture of cells; c) determining at least two dimensional coordinates of a cell of said first population of cells by reference to the captured image, and by reference to the presence of, or lack of, the label on said cell; and d) applying a lethal dose of energy to the coordinates of said cell. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification