Method for recovery of proteins prepared by recombinant DNA procedures

  • US 6,174,704 B1
  • Filed: 12/23/1998
  • Issued: 01/16/2001
  • Est. Priority Date: 12/23/1998
  • Status: Expired due to Term
First Claim
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1. In a method for the preparation and extraction of a protein of interest prepared by a recombinant DNA procedure from a host bacteria cell comprising expressing the protein in the host cell, lysing the cell to release the protein from the cell, and extracting the protein of interest from other host cellular components;

  • the improvement wherein the cell is lysed and the protein extracted with an aqueous reagent solution consisting essentially of an alkylglycoside or an alkylthioglycoside.

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