Method for the production and purification of adenoviral vectors

  • US 6,194,191 B1
  • Filed: 11/20/1997
  • Issued: 02/27/2001
  • Est. Priority Date: 11/20/1996
  • Status: Expired due to Term
First Claim
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1. A method for producing a purified adenovirus composition comprising:

  • a) growing host cells in a media;

    b) providing nutrients to said host cells by perfusion or through a fed-batch process;

    c) infecting said host cells with an adenovirus;

    d) lysing said host cells to provide a cell lysate comprising adenovirus, wherein said lysis is achieved through autolysis of infected cells; and

    e) purifying adenovirus from said lysate to provide a purified adenovirus composition.

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