Identification of congenital stationary night blindness in dogs

• US 6,201,114 B1
• Filed: 08/30/1999
• Issued: 03/13/2001
• Est. Priority Date: 10/06/1998
• Status: Expired due to Term

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Acland, Gregory M., Aguirre, Gustavo D., Ray, Kunal
• Primary Examiner(s): REYNOLDS, DEBORAH J
• Application Number:
  US09/385,259
• Time in Patent Office: 561 Days
• Field of Search: 536/23.1, 536/23.5, 435/6
• US Class Current: 536/23.5
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention relates to an isolated nucleic acid molecule encoding canine RPE65. The present invention also relates to an isolated nucleic acid molecule encoding canine RPE65 having a mutation in one or both alleles indicative of a carrier of or a dog affected with congenital stationary night blindness. The present invention also relates to a method for identifying dogs which are genetically normal are carriers of, or are affected with congenital stationary night blindness, said method including obtaining a biological sample from a dog and testing the biological sample for a gene encoding canine RPE65 having a nucleic acid mutation in one or both alleles indicative of a carrier of or a dog affected with congenital stationary night blindness. Another aspect of the present invention relates to a method for selecting dogs for breeding.

Claims

1. An isolated nucleic acid molecule encoding canine RPE65 and having a nucleotide sequence of SEQ. ID. No. 1.

2. An isolated nucleic acid molecule encoding canine RPE65 which has an amino acid sequence comprising SEQ. ID. No. 2.

3. An isolated nucleic acid molecule comprising a nucleotide sequence according to SEQ. ID. No. 1 having an AAGA deletion of nucleotides 487 through 490.