Modular assembly of antibody genes, antibodies prepared thereby and use

  • US 6,204,023 B1
  • Filed: 06/06/1995
  • Issued: 03/20/2001
  • Est. Priority Date: 11/01/1985
  • Status: Expired due to Term
First Claim
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1. A process for preparing an immunoglobulin that is capable of binding antigen, said immunoglobulin comprising (a) a heavy chain or heavy chain fragment and (b) a light chain or light chain fragment, said process comprising expressing, in a baceterial cell, of a first nucleic acid that encodes a bacterial signal sequence operably linked to either said heavy chain or to said heavy chain fragment, and a second nucleic acid that encodes a bacterial signal sequence operably linked to either said light chain or to said light chain fragment, such that the bacterial signal sequences bring about the transport of said heavy chain or said heavy chain fragment and said light chain or said light chain fragment through the cytoplasmic membrane of a bacterial cell, and, obtaining said immunoglobulin from the periplasmic space or culture medium, wherein said immunoglobulin is capable of binding antigen.

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