Biosensor

• US 6,212,417 B1
• Filed: 08/20/1999
• Issued: 04/03/2001
• Est. Priority Date: 08/26/1998
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro
• Primary Examiner(s): O'Connor, Cary
• Assistant Examiner(s): Natnithithadha, Navin
• Application Number:
  US09/379,128
• Time in Patent Office: 592 Days
• Field of Search: 600/345, 600/346, 600/347, 600/365, 600/395, 600/396, 600/397, 204/403
• US Class Current: 204/403.14
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A highly reliable biosensor is provided which is reduced in influences of any interfering substance or preexisting oxygen in a sample solution. The biosensor comprises an electrode system including a working electrode, a counter electrode and a third electrode also usable as an interfering substance detecting electrode, a reagent layer containing at least an oxidoreductase and an electron mediator, and an electrically insulating base plate for supporting the electrode system and the reagent layer, wherein the third electrode is arranged at an opposing position to that of at least one of the working electrode and the counter electrode and the reagent layer is arranged at a predetermined position apart from the third electrode.

Claims

1. A biosensor comprising:

an electrode system including a working electrode, a counter electrode and a third electrode which serves as an interfering substance detecting electrode,

a reagent layer containing at least an oxidoreductase and an electron mediator, and

an electrically insulating base plate for supporting said electrode system and said reagent layer,

wherein said third electrode is arranged at an opposing position to that of at least one of the working electrode and the counter electrode and said reagent layer is arranged at a predetermined position apart from the third electrode.

3. The biosensor in accordance with claim 1, wherein a layer essentially containing lecithin is arranged at a predetermined position avoiding said third electrode.

5. The biosensor in accordance with claim 1, wherein said reagent layer further contains a hydrophilic polymer.

2. A biosensor comprising:

an electrically insulating base plate,

an electrically insulating cover member for forming a sample solution supply pathway between the cover member and said base plate,

an electrode system including a working electrode, a counter electrode and a third electrode which serves as an interfering substance detecting electrode, and

a reagent layer

wherein said electrode system and said reagent layer are formed on said base plate or said cover member so as to be exposed to said sample solution supply pathway, and said third electrode is arranged at an opposing position to that of at least one of the working electrode and the counter electrode and said reagent layer is arranged at a position somewhere apart from the third electrode.

4. The biosensor in accordance with claim 2, wherein a layer essentially containing lecithin is arranged at a predetermined position avoiding said third electrode.

6. The biosensor in accordance with claim 2, wherein said reagent layer further contains a hydrophilic polymer.