Determination of an analyte in a liquid medium
First Claim
1. An electrochemical system for determining the presence of an analyte in a liquid medium, the system comprising:
- redox molecules comprising at least one first redox molecule and at least one second redox molecule, said first molecule transferring electrons between said electrode and said second molecule; and
an electrode having immobilized thereon a member of a recognition pair, said analyte being the other member of said pair, whereby presence of said analyte in the medium results in formation of one pair complex, being a complex between the immobilized member and said analyte;
formation of said pair complex said pair complex inhibiting the electron transfer and a change in the system'"'"'s electrical response as a result thereto, indicating presence of said analyte in medium.
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Accused Products
Abstract
Electrochemical determination of the presence, and optionally concentration of an analyte utilizes an electrode having immobilized thereon one member of a recognition pair, the recognition pair consisting of said analyte. In the presence of the analyte medium pair complexes, which are complexes between the analyte and the immobilized member are formed. The electrode forms part of an electrochemical system which includes also redox molecules comprising at least one first redox molecule and at least one second redox molecule, the former transferring electrons between the electrode and the latter. Upon formation of a pair complex the electron transfer is inhibited and the change in the electrical response then indicates the presence of said analyte in the medium.
44 Citations
10 Claims
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1. An electrochemical system for determining the presence of an analyte in a liquid medium, the system comprising:
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redox molecules comprising at least one first redox molecule and at least one second redox molecule, said first molecule transferring electrons between said electrode and said second molecule; and
an electrode having immobilized thereon a member of a recognition pair, said analyte being the other member of said pair, whereby presence of said analyte in the medium results in formation of one pair complex, being a complex between the immobilized member and said analyte;
formation of said pair complex said pair complex inhibiting the electron transfer and a change in the system'"'"'s electrical response as a result thereto, indicating presence of said analyte in medium. - View Dependent Claims (2, 3, 4, 5)
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6. An electrochemical method for determining presence of an analyte in a liquid medium, the method comprising:
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a) providing an electrode having immobilized thereon one member of a recognition pair, said analyte being the other member of said pair;
a first redox molecule not immobilized on the electrode, said first redox molecule being capable of transferring electrons between the electrode and a second redox molecule;
b) contacting said electrode with said liquid medium, whereby the presence therein of said analyte results in the formation of pair complexes, said pair complexes being complexes between the immobilized member and said analyte, on the surface said electrode;
c) contacting said electrode with said second redox molecule, and also with said first redox molecule; and
d) charging the electrode whereby electron transfer between said electrode and said second redox molecule by the mediation of said first redox molecule gives rise to an electrical response, a change in the electrical response following exposure of the electrode to said medium indicating the presence of said analyte in the medium. - View Dependent Claims (7, 8, 9, 10)
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Specification