Yeast vectors conferring antibiotic resistance

  • US 6,214,577 B1
  • Filed: 06/06/1995
  • Issued: 04/10/2001
  • Est. Priority Date: 05/22/1984
  • Status: Expired due to Term
First Claim
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1. An isolated DNA sequence comprising:

  • a) a gene encoding resistance to an antibiotic, wherein said antibiotic inhibits growth of an untransformed host yeast cell, b) a promotor sequence that is not naturally associated with said gene encoding antibiotic resistance, wherein said antibiotic resistance gene is transcribed from said promotor sequence, such that said DNA sequence provides for direct selection for said DNA sequence upon transformation into said host yeast cell, wherein the first selective medium encountered by the transformed cells subsequent to transformation contains said antibiotic, and wherein said promotor sequence is capable of transcribing said antibiotic resistant gene such that said isolated DNA provides for said direct selection upon integration of said DNA sequence into a chromosome of said host yeast cell, and c) a DNA sequence that is homologous to a portion of a chromosome of said host yeast cell.

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