Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions

  • US 6,221,581 B1
  • Filed: 06/07/1995
  • Issued: 04/24/2001
  • Est. Priority Date: 04/27/1984
  • Status: Expired due to Term
First Claim
Patent Images

1. A process for detecting a nucleic acid of interest comprising the steps of:

  • (a) providing one or more oligo- or polynucleotides, each comprising at least one single-stranded polynucleotide segment hybridizable with at least one portion of said nucleic acid of interest;

    (b) forming at least one first complex comprising said single-stranded polynucleotide segment or segments hybridized to said nucleic acid of interest;

    (c) contacting said first complex or complexes with one or more complex forming moieties which are capable of generating a signal to form a second complex or complexes;

    (d) capturing or collecting directly or indirectly said second complex or complexes;

    (e) optionally, washing to remove any uncaptured or uncollected elements; and

    (f) detecting directly or indirectly the presence of said nucleic acid of interest;

    wherein when said capturing or collecting is carried out with an oligo- or polynucleotide fixed or immobilized to a solid support, said oligo- or polynucleotide is substantially incapable of hybridizing with said nucleic acid of interest or portion thereof.

View all claims

    Thank you for your feedback