Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions

CAFC
  • US 6,221,581 B1
  • Filed: 06/07/1995
  • Issued: 04/24/2001
  • Est. Priority Date: 04/27/1984
  • Status: Expired due to Term
First Claim
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1. A process for detecting a nucleic acid of interest comprising the steps of:

  • (a) providing one or more oligo- or polynucleotides, each comprising at least one single-stranded polynucleotide segment hybridizable with at least one portion of said nucleic acid of interest;

    (b) forming at least one first complex comprising said single-stranded polynucleotide segment or segments hybridized to said nucleic acid of interest;

    (c) contacting said first complex or complexes with one or more complex forming moieties which are capable of generating a signal to form a second complex or complexes;

    (d) capturing or collecting directly or indirectly said second complex or complexes;

    (e) optionally, washing to remove any uncaptured or uncollected elements; and

    (f) detecting directly or indirectly the presence of said nucleic acid of interest;

    wherein when said capturing or collecting is carried out with an oligo- or polynucleotide fixed or immobilized to a solid support, said oligo- or polynucleotide is substantially incapable of hybridizing with said nucleic acid of interest or portion thereof.

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