Herbicide resistance in plants

• US 6,222,100 B1
• Filed: 06/05/1995
• Issued: 04/24/2001
• Est. Priority Date: 03/06/1984
• Status: Expired due to Term

• Current Assignee: M G I Pharma Incorporated
• Original Assignee: M G I Pharma Incorporated
• Inventors: Hibberd, Kenneth A., Anderson, Paul Curtis
• Primary Examiner(s): Fox, David T.
• Application Number:
  US08/464,157
• Time in Patent Office: 2,150 Days
• Field of Search: 47/58, 47/581, 800/235, 800/DIG.56, 800/DIG.55, 800/270, 800/300, 800/320, 800/320.1, 800/320.2, 800/320.3, 435/240.4, 435/240.5, 935/64
• US Class Current: 800/300
• CPC Class Codes: A01H 1/06 Processes for producing mutations, e.g. treatment with chemicals or with radiation specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 ; process for producing transgenic plants C12N15/82
• CPC Class Codes: A01H 1/124 to sulfonylurea
• CPC Class Codes: A01H 5/10 Seeds
• CPC Class Codes: A01H 6/46 Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
• CPC Class Codes: C12N 15/01 Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 15/8278 Sulfonylurea
• CPC Class Codes: C12N 5/04 Plant cells or tissues culture media C12N5/0025
• CPC Class Codes: C12N 9/88 Lyases (4.)

Abstract

This invention is directed to the production of plants, plant tissues and seeds which are resistant to inhibition by an herbicide which normally inhibits the growth and development of those plants, plant tissues and plant seeds. In particular this invention is directed to altered acetohydroxyacid synthase enzymes which are resistant to inhibition by herbicides which normally inhibit the activity of the synthase before such alteration. This invention further relates to genes encoding such enzymes, and to processes for utilizing these novel genes and enzymes. Further products of the invention include plants, plant tissues and seeds which exhibit resistance to such herbicides resulting from expression of genes encoding herbicide resistant acetohydroxyacid synthase enzyme.

Claims

1. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by a 2-(2-imidazolin-2-yl)pyridine herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, in the presence of said herbicide or derivative at levels which normally inhibit the growth of that species of plant.

2. The method according to claim 1, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 263,499.

3. The method according to claim 1, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 243,997.

4. The method according to claim 1, 2 or 3, wherein the plant is a cereal.

5. The method according to claim 1, 2 or 3, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

37. The method according to claim 1, 2 or 3, wherein the plant is rice.

6. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by a 2-(2-imidazolin-2-yl)quinoline herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, in the presence of said herbicide or derivative at levels which normally inhibit the growth of that species of plant.

7. The method according to claim 6, wherein the 2-(2-imidazolin-2-yl)quinoline herbicide is AC 252,214.

8. The method according to claim 6 or 7, wherein the plant is a cereal.

9. The method according to claim 6 or 7, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

38. The method according to claim 6 or 7, wherein the plant is rice.

10. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by an herbicidal sulfonamide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, in the presence of said herbicide or derivative at levels which normally inhibit the growth of that species of plant.

11. The method according to claim 10, wherein the herbicidal sulfonamide is 2-chloro-N[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl]benzenesulfonamide.

12. The method according to claim 10 or 11, wherein the plant is a cereal.

13. The method according to claim 10 or 11, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

39. The method according to claim 10 or 11, wherein the plant is rice.

14. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by a 1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, in the presence of said herbicide or derivative at levels which normally inhibit the growth of that species of plant.

15. A method according to claim 14, wherein the 1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide herbicide is 5,7-dimethyl-N-(2,6-dichlorophenyl)-1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide.

16. The method according to claim 14 or 15, wherein the plant is a cereal.

17. The method according to claim 14 or 15, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

40. The method according to claim 14 or 15, wherein the plant is rice.

18. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by a 2-(2-imidazolin-2-yl)pyridine herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, and wherein the growth of said plant is sensitive to inhibition by an herbicidal sulfonamide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase of that species of plant, in the presence of said 2-(2-imidazolin-2-yl)pyridine herbicide or derivative thereof at levels which normally inhibit the growth of that species of plant.

19. A method according to claim 18, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 263,499.

20. A method according to claim 18, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 243,997.

21. The method according to claim 18, 19 or 20, wherein the plant is a cereal.

22. The method according to claim 18, 19 or 20, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

23. A method according to claim 18, 19 or 20, wherein the herbicidal sulfonamide is 2-chloro-N[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl]benzenesulfonamide.

24. The method according to claim 23, wherein the plant is a cereal.

25. The method according to claim 23, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

41. The method according to claim 18, 19 or 20, wherein the plant is rice.

42. The method according to claim 23, wherein the plant is rice.

26. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by a 2-(2-imidazolin-2-yl)pyridine herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide or derivative at levels of said herbicide or derivative which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, and wherein the growth of said plant is sensitive to inhibition by a 1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide herbicide, or an herbicidal derivative thereof, at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase of that species of plant, in the presence of said 2-(2-imidazolin-2-yl)pyridine herbicide or derivative at levels which normally inhibit the growth of that species of plant.

27. A method according to claim 26, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 263,499.

28. A method according to claim 26, wherein the 2-(2-imidazolin-2-yl)pyridine herbicide is AC 243,997.

29. The method according to claim 26, 27 or 28, wherein the plant is a cereal.

30. The method according to claim 26, 27 or 28, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

31. A method according to claim 26, 27 or 28, wherein the 1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide herbicide is 5,7-dimethyl-N-(2,6-dichlorophenyl)-1,2,4-triazolo[1,5-a]pyrimidine-2-sulfonamide.

32. The method according to claim 31, wherein the plant is a cereal.

33. The method according to claim 31, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

43. The method according to claim 26, 27 or 28, wherein the plant is rice.

44. The method according to claim 31, wherein the plant is rice.

34. A method for growing a monocotyledonous plant, the growth of which is resistant to an herbicide, comprising:

cultivating a monocotyledonous plant, the growth of which is resistant to inhibition by an herbicide at levels which normally inhibit the growth of that species of plant by inhibiting the activity of acetohydroxyacid synthase, wherein said resistance is conferred by an altered acetohydroxyacid synthase whose activity is resistant to inhibition by said herbicide at levels of said herbicide which normally inhibit the activity of an unaltered acetohydroxyacid synthase of that species of plant, in the presence of said herbicide at levels which normally inhibit the growth of that species of plant.

35. The method according to claim 34, wherein the plant is a cereal.

36. The method according to claim 34, wherein the plant is selected from the group consisting of wheat, barley, sorghum, oats, rye and millet.

45. The method according to claim 34, wherein the plant is rice.