Optochemical sensor

• US 6,254,829 B1
• Filed: 09/28/1998
• Issued: 07/03/2001
• Est. Priority Date: 10/03/1997
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Original Assignee: AVL Medical Instruments
• Inventors: Hartmann, Paul, Leiner, Marco Jean Pierre
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Handy, Dwayne K.
• Application Number:
  US09/161,841
• Time in Patent Office: 1,009 Days
• Field of Search: 422/55, 422/57, 422/82.05, 422/82.06, 436/68, 436/136, 436/172
• US Class Current: 422/82.06
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 2021/7763 Sample through flow
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: Y10T 436/20 Oxygen containing
• CPC Class Codes: Y10T 436/207497 Molecular oxygen

Abstract

There is disclosed an optical sensor including a matrix containing a luminescence indicator whose luminescence may be quenched by oxygen. The optical sensor contains at least one agent capable of deactivating singlet oxygen and has an enhanced stability relative to oxygen.

Claims

1. An optical sensor configuration comprising:

an optical sensor including a polymer matrix, the matrix including a luminescence indicator contained in said matrix wherein said optical sensor comprises at least one agent capable of deactivating singlet oxygen, and a transparent carrier,

a light source for exciting the luminescence indicator, and

a detector for determining at least one optical property of said luminescence indicator in the presence of a sample.

2. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen comprises at least one amino group.

3. An optical sensor configuration as set forth in claim 2, wherein said amino group is a tertiary amino group.

4. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen is a hindered amine light stabilizer (HALS).

5. An optical sensor configuration as set forth in claim 1, wherein the agent capable of deactivating singlet oxygen is a transition metal complex.

6. An optical sensor configuration as set forth in claim 5, wherein said transition metal complex is a complex of a dialkyldithio carbamate and a transition metal ion.

7. An optical sensor configuration as set forth in claim 5, wherein said transition metal complex is a complex of a dialkyldithio phosphate and a transition metal ion.

8. An optical sensor configuration as set forth in claim 5, wherein said transition metal complex is a complex of a Schiff'"'"'s base and a transition metal ion.

9. An optical sensor configuration as set forth in claim 6, 7 or 8, wherein the transition metal ion is selected from the group consisting of Ni(II), Co(II), Zn(II) and Fe(III).

10. An optical sensor configuration as set forth in claim 1, wherein the agent capable of deactivating singlet oxygen is a salt with an anion selected from the group consisting of Cl⁻

, Br⁻

,I and NO₃⁻

.

11. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen is a carotenoid.

12. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen is contained in said matrix.

13. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen is bound to said matrix.

14. An optical sensor configuration as set forth in claim 1, wherein said agent capable of deactivating singlet oxygen is bound to said luminescence indicator.

15. An optical sensor configuration as set forth in claim 14, wherein said agent capable of deactivating singlet oxygen is bound to said luminescence indicator via a group —

(CH₂)_n—

, wherein n is an integer from 3 to 20.

16. An optical sensor as set forth in claim 1, wherein said polymer is selected from the group consisting of polystyrene, polyvinylchloride, plasticized polyvinylchloride, polymethylmethacrylate, plasticized polymethylmethacrylate, a polymethylmethacrylate/cellulose-acetyl-butyral mixture, a silica gel, a sol gel, a hydrogel, a silicone, poly-α

-methylstyrene, a polysulfone, ethyl cellulose, cellulose triacetate, polytetrafluoroethylene, a polyester, polybutadiene, polystyrene-co-butadiene, polyurethane, polyvinylbutyral, polyethylacrylate and poly-2-hydroxyethylmethacrylate.

17. An optical sensor as set forth in claim 1, wherein said polymer is prepared of monomers at least partially carrying an amino group.

18. An optical sensor as set forth in claim 17, wherein said amino group is a tertiary amino group.

19. An optical sensor as set forth in claim 1, wherein said luminescence indicator is selected from the group consisting of a tris-chelate complex of ruthenium(II), a tris-chelate complex of osmium(II), a tris-chelate complex of rhodium(III), a tris-chelate complex of iridium(III), a metal porphyrin of platinum, a metal porphyrin ketone of platinum, a metal porphyrin of palladium, a metal porphyrin ketone of palladium, a lanthanid complex of terbium and a polycyclic aromatic hydrocarbon.

20. An optical sensor comprising:

an optical sensor including a polymer matrix, a luminescence indicator contained in said matrix wherein said matrix comprises at least one agent capable of deactivating singlet oxygen, and

a transparent carrier.

21. A method for determining an analyte in a gaseous liquid or sample which method comprises:

providing an optical sensor including a polymer matrix, a luminescence indicator contained in said matrix and exhibiting a luminescence capable of being quenched by oxygen thus forming singlet oxygen, and at least one agent capable of deactivating singlet oxygen for stabilizing said luminescence indicator and said matrix,

providing a light source for generating light for exciting said luminescence indicator,

directly contacting said sample with said sensor, and

providing measuring means for measuring at least one optical property of said luminescence indicator.

22. A method for determining an analyte contained in a gaseous sample, which method comprises:

providing an optical sensor including a polymer matrix, a luminescence indicator contained in said matrix and exhibiting a luminescence capable of being quenched by oxygen thus forming singlet oxygen, and at least one agent capable of deactivating singlet oxygen for stabilizing said luminescent indicator and said matrix, and a transparent carrier,

providing a light source for generating light for exciting said luminescence indicator,

indirectly contacting said sample with said sensor and determining the presence of the analyte, and

providing measuring means for measuring at least one optical property of said luminescence indicator.