Purification of biologically active peptides from milk

  • US 6,268,487 B1
  • Filed: 05/13/1996
  • Issued: 07/31/2001
  • Est. Priority Date: 05/13/1996
  • Status: Expired due to Term
First Claim
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1. A method of separating an exogenous component from a milk sample, comprising:

  • a) subjecting the milk sample containing at least one of the following;

    fat, casein micelles, somatic cells or particulate matter, to tangential flow filtration across a membrane of sufficient porosity to form a retentate and a permeate comprising the exogenous component;

    b) subjecting the permeate to a chromatography capture device to remove the exogenous component and providing an effluent;

    c) combining the effluent with the retentate; and

    d) repeating Steps a) through c) at least once and until the exogenous component is free of fat, casein micelles, somatic cells or particulate matter, and wherein the recovery of the exogenous component is 75-90%.

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