DNA sequence of a gene of hydroxy-phenyl pyruvate dioxygenase and production of plants containing a gene of hydroxy-phenyl pyruvate dioxygenase and which are tolerant to certain herbicides

• US 6,268,549 B1
• Filed: 02/18/1998
• Issued: 07/31/2001
• Est. Priority Date: 06/02/1995
• Status: Expired due to Term

• Current Assignee: Bayer SAS (Bayer AG)
• Original Assignee: Aventis Cropscience SA
• Inventors: Rolland, Anne, Sailland, Alain, Pallett, Ken, Matringe, Michel
• Primary Examiner(s): Benzion, Gary
• Application Number:
  US08/945,515
• Time in Patent Office: 1,259 Days
• Field of Search: 800/295, 800/298, 800/278, 800/275, 536/23.5, 536/23.6, 536/23.7, 536/24.1, 435/468, 435/419, 435/320.1
• US Class Current: 800/295
• CPC Class Codes: C12N 15/8209 Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Abstract

An isolated gene from Pseudomonas is described which expresses a hydoxy phenyl pyruvate dioxygenase. Also described are chimeric genes for introduction into plants to overexpress a hydoxy phenyl pyruvate dioxygenase and produce plants which are tolerant to herbicides.

Claims

1. An isolated gene which expresses a hydroxyphenylpyruvate dioxygenase (HPPD) from Pseudomonas.

2. Sequence according to claim 1, characterized in that it is from Pseudomonas fluorescens.

3. A chimeric gene comprising, in the direction of transcription:

at least one promoter regulation sequence from a gene expressing itself naturally in plants,

a heterologous coding sequence under control of the promoter regulation sequence, and

at least one polyadenylation sequence, characterized in that the coding sequence expresses hydroxyphenylpyruvate diogenase (HPPD).

4. Chimeric gene according to claim 3, characterized in that the promoter regulation sequence comprises at least one histone promoter.

5. Chimeric gene according to claim 3, characterized in that it comprises, between the promoter regulation sequence and the coding sequence, a transit peptide.

6. Chimeric gene according to claim 3, characterized in that it comprises, between the promoter regulation sequence and the coding sequence and, in the transcription direction, a sequence coding for a transit peptide of a plant gene coding for a plastid localization enzyme, a part sequence of the N-terminal mature part of a plant gene coding for a plastid localization enzyme, then a sequence coding for a second transit peptide of a plant gene coding for a plastid localization enzyme.

7. Chimeric gene according to claim 3, characterized in that it comprises, between the promoter regulation sequence and the coding sequence, a sequence of a transcription activator (enhancer).

8. A vector comprising a chimeric gene according to claim 3.

9. A plant which contains the chimeric gene as claimed in claim 3.

10. A method of selective herbicidal treatment of plants, which comprises applying an HPPD-inhibiting herbicide to the plant as claimed in claim 9.

11. The method of claim 10 wherein the herbicide is an isoxazole.

12. The method of claim 11, wherein the isoxazole is4-[4-CF₃-2-methylsulfonylbenzoyl]-5-cyclopropyl isoxazole.

13. The method of claim 10 wherein the herbicide is a diketonitrile.

14. The method of claim 10 wherein the herbicide is a triketone.

15. The method of claim 10 wherein the herbicide is a sulcotrione.