Assay for the identification of IgE antibody suppressors

  • US 6,270,746 B1
  • Filed: 02/17/1999
  • Issued: 08/07/2001
  • Est. Priority Date: 02/17/1999
  • Status: Expired due to Fees
First Claim
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1. A screening method for identifying agents having IgE suppressor activity, the method comprising:

  • (a) determining the IgE responsiveness to antigen of an animal before and after administration to the animal of a candidate IgE suppressor, wherein the animal has been treated with an IgE enhancer prior to performance of the screening method, the steps for determining such IgE responsiveness comprising;

    (i) sensitizing the animal to a sensitizing antigen within a day of treatment of the animal with the IgE enhancer;

    (ii) delivering a primary immunization dose of an antigen to the animal, wherein the antigen is immunologically related to the sensitizing antigen;

    (iii) measuring levels of IgE in the animal;

    (iv) delivering the candidate IgE suppressor to the animal; and

    , (v) measuring levels of IgE in the animal;

    then, (b) comparing the measurements obtained in step (a)(iii) with the measurements obtained in step (a)(v), wherein a decline in any of the measured values so compared indicates that the candidate IgE suppressor suppresses the production and/or activity of IgE in the animal.

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