Method of preparing suppressor T cells with allogeneic mesenchymal stem cells

• US 6,281,012 B1
• Filed: 05/01/2000
• Issued: 08/28/2001
• Est. Priority Date: 10/12/1999
• Status: Expired due to Term

• Current Assignee: Mesoblast International Srl
• Original Assignee: Osiris Therapeutics Incorporated (Smith & Nephew PLC)
• Inventors: McIntosh, Kevin, Klyushnenkova, Elena
• Primary Examiner(s): Nolan, Patrick J.
• Assistant Examiner(s): Ewoldt, Gerald R.
• Application Number:
  US09/563,768
• Time in Patent Office: 484 Days
• Field of Search: 424/93.71, 424/534, 435/346, 435/347, 435/372.3, 435/373, 435/325, 435/363, 435/366, 435/377
• US Class Current: 435/347
• CPC Class Codes: A61K 2035/122 for inducing tolerance or supression of immune responses
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2039/5158 Antigen-pulsed cells, e.g. T-cells
• CPC Class Codes: A61K 2239/38 characterised by the dose, timing or administration schedule
• CPC Class Codes: A61K 39/001 Preparations to induce tolerance to non-self, e.g. prior to transplantation
• CPC Class Codes: A61K 39/4611 T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
• CPC Class Codes: A61K 39/4621 immunosuppressive or immunotolerising
• CPC Class Codes: A61K 39/46434 Antigens related to induction of tolerance to non-self
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: C12N 2502/1358 Bone marrow mesenchymal stem cells (BM-MSC)
• CPC Class Codes: C12N 5/0637 Immunosuppressive T lymphocytes, e.g. regulatory T cells or Treg

Abstract

A method of reducing an immune response to a transplant in a recipient by treating said recipient with an amount of suppressor T cells effective to reduce or inhibit host rejection of the transplant. The suppressor T cells can be administered before, at the same time as, or after the transplant. Also disclosed is a method of inducing a reduced immune response against a host by foreign tissue, i.e., graft versus host disease, by treatment with suppressor T cells.

Claims

1. A method of preparing suppressor T-cells, comprising:

contacting activated immune effector T-Cells with allogeneic mesenchymal stem cells in culture to produce suppressor T-cells; and

separating said suppressor T-cells from said culture.

2. The method of claim 1 wherein the immune effector T-cells are CD8+ cells.