Apparatus and method for transferring liquids
First Claim
1. A device comprising:
- (a) a first plate having a plurality of transfer elements, each transfer element comprising an aperture in said plate wherein said aperture is capable of being electrically activated;
(b) a multiwell plate comprising a plurality of wells;
(c) one or more attaching elements on a first side of said first plate for sealingly attaching said first plate to said multiwell plate to form a sealed system except for said apertures;
(d) electrodes for electrically activating said aperture.
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Accused Products
Abstract
The present invention concerns devices, apparatus and methods for transferring liquids. One aspect of the present invention is a device comprising a plate having a plurality of transfer elements. Each of the transfer elements comprises an aperture in the plate where the aperture is capable of being electrically activated. The plate has one of more attaching elements for attaching the plate to a multiwell plate to form a sealed system except for the apertures of the transfer elements. Usually, the device is adapted for sealing attachment to a multiwell plate. In a method in accordance with the present invention a quantity of liquid is disposed to a second side of a plate having a plurality of apertures in the plate. The apertures are capable of being electrically activated. The liquid is present in a closed well except for the apertures in the plate. To simultaneously expel liquid from the apertures, the apertures are electrically activated. Also disclosed are kits comprising a device in accordance with the present invention.
454 Citations
35 Claims
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1. A device comprising:
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(a) a first plate having a plurality of transfer elements, each transfer element comprising an aperture in said plate wherein said aperture is capable of being electrically activated;
(b) a multiwell plate comprising a plurality of wells;
(c) one or more attaching elements on a first side of said first plate for sealingly attaching said first plate to said multiwell plate to form a sealed system except for said apertures;
(d) electrodes for electrically activating said aperture. - View Dependent Claims (2, 3, 4, 32, 33, 34, 35)
(a) the device of claim 1 and (b) a sample receiving plate.
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33. The kit of claim 32 wherein said sample receiving plate is selected from the group consisting of planar surfaces and sample receiving reservoirs.
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34. The kit of claim 32 which comprises one or more reagents for conducting a chemical synthesis or an analysis.
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35. The kit of claim 32 which comprises a multiwell plate.
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5. An apparatus for transferring liquid, said apparatus comprising:
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(a) a first plate comprising a plurality of individual wells containing said liquid, said wells being formed in said first plate on a first side thereof, wherein a second side of said first plate is free of openings;
(b) a second plate comprising a first side and a second side and a plurality of transfer elements, each transfer element comprising an aperture in said second plate, wherein said apertures are capable of being electrically activated, said second plate being adapted for simultaneously transferring precise amounts of a liquid from said first plate to a sample receiving plate by electrically activating said apertures, said second side of said second plate being sealingly attached to the first side of said first plate; and
(c) electrodes for electrically activating said aperture. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for trnsfering liquid, said method comprising:
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(a) disposing a quantity of liquid to a second side of a plate having a plurality of apertures therein that are capable of being electrically activated, wherein said liquid is present in a closed container comprising individual wells of a multiwell plate and (b) electrically activating said apertures. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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21. A method for transferring liquid, said method comprising:
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(a) providing a first plate comprising a plurality of individual wells containing said liquid, said wells being formed in said first plate on a first side thereof, (b) providing a second plate comprising a first side and a second side and a plurality of transfer elements, each transfer element comprising an aperture in said second plate, wherein said apertures are capable of being electrically activated, said second plate being adapted for simultaneously transferring precise amounts of a liquid from said first plate to a sample receiving plate by electrically activating said apertures, (c) sealingly attaching said second side of said second plate to the first side of said first plate, (d) positioning said apertures adjacent to an array of sample receiving plate, and (e) electrically activating said apertures. - View Dependent Claims (22, 23, 24, 25, 26, 27)
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28. A method for transferring liquid, said method comprising:
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(a) attaching, to a multiwell plate having liquid contained in the wells thereof, a second plate comprising a first side and a second side and a plurality of apertures therethrough, each of said apertures being at least partially comprised of an electroconductive material, each of said apertures being aligned with a corresponding well of said multiwell plate, said second plate comprising on said second side one or more attaching elements and said second plate being attached to the top of said multiwell plate by said attaching element, (b) inverting said multiwell plate attached to said second plate so that liquid is disposed at each of said apertures, the dimensions of said apertures being such that liquid does not exit said apertures, (c) positioning said apertures adjacent to an array of sample receiving reservoirs of a microfluidic network in a third plate wherein each of said microfluidic networks has an electrode connected to an electrode adjacent one of said apertures, (d) applying an electric potential across said electrodes causing a portion of said liquid to exit said apertures and enter a corresponding sample receiving reservoir. - View Dependent Claims (29, 30, 31)
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Specification