Methods for producing secreted ligand-binding fusion proteins

  • US 6,300,099 B1
  • Filed: 05/30/2000
  • Issued: 10/09/2001
  • Est. Priority Date: 01/22/1988
  • Status: Expired due to Fees
First Claim
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1. A method for producing a secreted ligand-binding fusion protein comprising:

  • (a) introducing into a host cell a DNA construct comprising a transcriptional promoter operatively linked to a secretory signal sequence followed downstream by and in proper reading frame with a DNA sequence encoding a polypeptide chain that comprises (i) a ligand-binding domain of a receptor, wherein the receptor is a receptor for a growth factor or a hormone, and (ii) an immunoglobulin constant region polypeptide; and

    (b) culturing the host cell in an appropriate culture medium whereby the ligand-binding fusion protein is secreted, wherein the ligand-binding fusion protein binds the growth factor or the hormone.

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