Non-invasive measurement of blood component using retinal imaging

• US 6,305,804 B1
• Filed: 03/24/2000
• Issued: 10/23/2001
• Est. Priority Date: 03/25/1999
• Status: Expired due to Fees

• Current Assignee: Fovioptics Incorporated
• Original Assignee: Fovioptics Incorporated
• Inventors: Routt, Wilson, Rice, Mark J., Williams, William T., Rioux, James M., Sweat, Robert H. Jr.
• Primary Examiner(s): MANUEL, GEORGE C
• Application Number:
  US09/534,782
• Time in Patent Office: 578 Days
• Field of Search: 351/200, 351/221, 600/318, 600/319, 600/320, 600/315, 600/316, 600/323, 600/328, 424/9.6, 356/41, 356/40
• US Class Current: 351/221
• CPC Class Codes: A61B 3/1233 for measuring blood flow, e.g. at the retina
• CPC Class Codes: A61B 3/145 by video means
• CPC Class Codes: A61B 5/0013 Medical image data A61B1/00011, A61B6/56, A61B8/56 take precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters

Abstract

Illuminating light of selected wavelengths in the visible or infrared range is projected through the pupil of the eye onto the fundus, and the light reflected back and out through the pupil is detected and analyzed, preferably using the area of the optic disk for analyzing the retinal vessels overlying the optic disk. Specific wavelengths of illuminating light may be chosen for each blood component to be analyzed depending on the spectral characteristics of the substance being analyzed. The reflected image from the retina may be used to measure non-photoreactive blood components such as hemoglobin, and photoreactive components such as bilirubin. For the measurement of photoreactive components, images may be taken before and after, or during, illumination of the eye with light at wavelengths which will affect the photoreactive analyte, enabling measurements of the concentration of the analyte.

Claims

1. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component.

2. The method of claim 1 wherein the selected blood component is hemoglobin.

3. The method of claim 1 wherein the selected blood component is glucose.

4. The method of claim 1 wherein in the step of analyzing the wavelength components, only light from the regions of the image corresponding to blood vessels is analyzed.

5. The method of claim 1 wherein the light projected into the eye contains wavelengths in the visible or near infrared range and wherein detecting the light reflected from the fundus includes detecting the light in the visible or near infrared range.

6. The method of claim 1 wherein the step of forming an image forms an image of the blood vessels in the region overlying the optic disk of the eye and wherein the step of analyzing the wavelength components analyzes the light reflected from the optic disk.

7. The method of claim 1 wherein the step of projecting light into the eye is carried out by projecting light through the pupil of the eye onto the fundus.

8. The method of claim 1 wherein the step of projecting light into the eye is carried out by projecting light taking a path at an angle into the eye just lateral to the cornea, near the corneoscleral junction.

9. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component, wherein the selected blood component is bilirubin.

10. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component;

(d) sensing the blood flow of the individual being tested to detect systole and wherein this information is used to time the step of detecting the light reflected from the fundus and forming an image.

13. The method of claim 10 wherein the blood component is hemoglobin and the multiple images are filtered to pass light centered at wavelengths of 640 nm, 766 nm and 800 nm.

11. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component, wherein multiple images are formed of the fundus at discrete times over a period of time and including selecting an image from the multiple images and analyzing that image to determine the concentration of the blood component.

12. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component, wherein the image is split into multiple images that are passed through filters that pass different wavelengths to provide multiple filtered images, and analyzing the multiple filtered images to determine the concentration of the blood component.

14. A method for the detection of the concentration of a blood component in an individual comprising:

(a) projecting light having selected wavelengths into an eye of the individual to illuminate the fundus;

(b) detecting the light reflected from the fundus and forming an image of a portion of the fundus containing blood vessels;

(c) analyzing selected wavelength components in the detected light from the blood vessels in the image to determine the concentration of a selected blood component;

(d) transmitting data corresponding to the image over a communications link to a remote location, and performing the step of analyzing selected wavelength components at the remote location.

15. A method of detecting the concentration of a photoreactive analyte in the blood of an individual comprising:

(a) projecting light into the eye of the individual to illuminate the fundus that contains wavelengths not including light at wavelengths that will break down the photoreactive analyte and detecting the light reflected from the fundus to determine the intensity of the reflected light at these wavelengths;

(b) projecting light into the eye to illuminate the fundus containing wavelengths that break down the photoreactive analyte, and projecting light into the eye to illuminate the fundus containing the wavelengths that do not break down the photoreactive analyte and detecting the intensity of such light reflected from the fundus; and

(c) determining the difference between the intensity of light detected with no wavelengths that break down the photoreactive analyte projected on the fundus and the intensity of light detected when light which breaks down the photoreactive analyte has been projected onto the fundus, from which the analyte concentration may be determined.

16. The method of claim 15 wherein the target analyte is bilirubin or an associated molecule.

17. The method of claim 16 wherein in the steps of projecting light that do not break down bilirubin, the light projected does not contain wavelengths in the range of 470 nm±

30 mn.

18. The method of claim 17 wherein the light projected that does not break down bilirubin contains light centered at 550 nm.

19. The method of claim 17 wherein the step of projecting light that breaks down bilirubin comprises projecting light having wavelengths in the range of 470 nm±

30 nm.

20. The method of claim 16 wherein step (b) includes projecting light containing wavelengths at 470 nm and at 550 nm simultaneously.

21. The method of claim 16 wherein step (b) is carried out by projecting light at 470 nm followed thereafter by projecting light containing wavelengths at 550 nm to illuminate the fundus, and wherein the light at 550 nm reflected from the fundus is detected.