DNA sequencing by parallel oligonucleotide extensions

  • US 6,306,597 B1
  • Filed: 03/29/1999
  • Issued: 10/23/2001
  • Est. Priority Date: 04/17/1995
  • Status: Expired due to Term
First Claim
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1. A method for identifying a sequence of nucleotides in a polynucleotide, the method comprising the steps of:

  • (a) extending an initializing oligonucleotide along the polynucleotide by ligating an oligonucleotide probe thereto to form an extended duplex;

    (b) identifying one or more nucleotides of the polynucleotide; and

    (c) repeating steps (a) and (b) until the sequence of nucleotides is determined.

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