Methods for monitoring a physiological analyte
First Claim
1. A method for monitoring a physiologic analyte in a subject, comprising steps of:
- (a) collecting the analyte in a collection reservoir, wherein (i) said collection reservoir is in contact with the subject such that the analyte can move from the subject into said collection reservoir, and (ii) the collection reservoir is in operative contact with a detector;
(b) contacting the analyte in the collection reservoir with an enzyme that reacts with the analyte to produce a detectable chemical species, wherein the reaction of the analyte and the enzyme further results in conversion of the analyte to a reaction product that is not detected by the detector;
(c) using the detector to detect the detectable chemical species in the collection reservoir, and once the detectable chemical species has been detected, rendering the detectable chemical species undetectable by the detector; and
(d) repeating steps (a), (b) and (c).
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Abstract
A method for continual monitoring of a physiologic analyte in a subject includes steps of contacting the subject with a collection reservoir such that the analyte can move from the subject into the collection reservoir, the contents of the collection reservoir being in operative communication with a detector, collecting the analyte in the collection reservoir, using the detector to detect the analyte in the collection reservoir, and, once the analyte has been detected, rendering the analyte undetectable by the detector. Also, apparatus for continual monitoring of a physiologic analyte in a subject includes a collection reservoir for receiving the analyte from the subject, the contents of the collection reservoir being in operative relationship with a detector that detects the analyte in the reservoir, and means for rendering the analyte, once detected, undetectable by the reservoir.
659 Citations
39 Claims
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1. A method for monitoring a physiologic analyte in a subject, comprising steps of:
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(a) collecting the analyte in a collection reservoir, wherein (i) said collection reservoir is in contact with the subject such that the analyte can move from the subject into said collection reservoir, and (ii) the collection reservoir is in operative contact with a detector;
(b) contacting the analyte in the collection reservoir with an enzyme that reacts with the analyte to produce a detectable chemical species, wherein the reaction of the analyte and the enzyme further results in conversion of the analyte to a reaction product that is not detected by the detector;
(c) using the detector to detect the detectable chemical species in the collection reservoir, and once the detectable chemical species has been detected, rendering the detectable chemical species undetectable by the detector; and
(d) repeating steps (a), (b) and (c). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for monitoring a physiologic analyte in a subject, comprising steps of:
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(a) collecting the analyte in a collection reservoir by applying energy to a skin surface of the subject, wherein (i) said collection reservoir is in contact with the skin surface of the subject such that the analyte can move from the subject into said collection reservoir, and (ii) the collection reservoir is in operative contact with a detector;
(b) contacting the analyte in the collection reservoir with an enzyme that reacts with the analyte to produce a detectable chemical species, wherein the reaction of the analyte and the enzyme further results in conversion of the analyte to a reaction product that is not detected by the detector;
(c) using the detector to detect the detectable chemical species in the collection reservoir, and once the detectable chemical species has been detected, rendering the detectable chemical species undetectable by the detector; and
(d) repeating steps (a), (b) and (c). - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for monitoring a physiologic analyte in a subject, wherein the method comprises the steps of:
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(a) collecting the analyte in a collection reservoir by applying energy to a skin surface of the subject, wherein (i) said collection reservoir is in contact with the skin surface of the subject such that the analyte can move from the subject into said collection reservoir, and (ii) the collection reservoir is in operative contact with a detector;
(b) discontinuing collection of the analyte by discontinuing the application of energy, while the collection reservoir remains in contact with the skin surface of the subject;
(c) contacting the analyte in the collection reservoir with an enzyme that reacts with the analyte to produce a detectable chemical species, wherein (i) the reaction of the analyte and the enzyme further results in conversion of the analyte to a reaction product that is not detected by the detector, and (ii) said contacting of the analyte is carried out while the collection reservoir remains in contact with the skin surface of the subject;
(d) detecting the detectable chemical species in the collection reservoir using the detector, and once the detectable chemical species has been detected, rendering the detectable chemical species undetectable by the detector, said detecting performed while the collection reservoir remains in contact with the skin surface of the subject;
(e) discontinuing detection after substantially all the detectable chemical species has been detected and rendered undetectable, thereby preventing significant accumulation of detectable chemical species in the collection reservoir; and
sequentially repeating steps (a), (b), (c), (d), and (e) while the collection reservoir remains in contact with the skin surface of the subject. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification