Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of dioxetanes

  • US 6,322,727 B1
  • Filed: 03/29/2000
  • Issued: 11/27/2001
  • Est. Priority Date: 07/24/1986
  • Status: Expired due to Term
First Claim
Patent Images

1. A kit for detecting a first substance in a sample, comprising:

  • (1) a stabilized 1,2dioxetane bearing an enzyme-labile substituent, which is destabilized and caused to decompose by contacting said 1,2-dioxetane with an enzyme under conditions which cause said enzyme to cleave said enzyme-labile group from said dioxetane, yielding a negatively charged oxygen anion bonded to said 1,2-dioxetane, which causes said 1,2-dioxetane to decompose without input from an external excitation energy source, said decomposition being accompanied by chemiluminescence; and

    (2) a second component selected from the group consisting of said enzyme and said enzyme conjugated to a specific affinity substance;

    wherein said specific affinity substance is selected from the group consisting of an antigen, an antibody and a nucleic acid probe.

View all claims

    Thank you for your feedback