Thin-well microplate and methods of making same

• US 6,340,589 B1
• Filed: 07/19/2000
• Issued: 01/22/2002
• Est. Priority Date: 07/23/1999
• Status: Expired due to Term

• Current Assignee: Eppendorf AG
• Original Assignee: MJ Research Incorporated (Bio-Rad Laboratories Incorporated)
• Inventors: Turner, Bruce R., Titus, David E.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/619,116
• Time in Patent Office: 552 Days
• Field of Search: 422/102, 422/287.2, 435/288.4, 435/305.2, 435/305.3
• US Class Current: 435/287.2
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/163 Biocompatibility
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B29C 45/16 Making multilayered or multicoloured articles B29C45/0062 takes precedence; feeding colouring materials into the injection unit B29C45/1816
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

A thin-well microplate and methods of manufacturing same are provided, wherein the thin-well microplate is conducive for use with automated equipment, such as robotic handling equipment, and in high temperature procedures, such as thermal cycling. The thin-well microplate is constructed of two components including a skirt and frame portion and a well and deck portion with a plurality of sample wells; both portions being joined to form a unitary microplate. Each of the skirt and frame and well and deck portions is constructed of a suitable material that imparts a specific combination of physical and material properties to the thin-well microplate. The skirt and frame portion and the well and deck portion are each constructed of the material that will allow each portion to withstand high temperature conditions and use of automated equipment, while retaining the physical characteristics each portion requires for optimal performance of the thin-well microplate. Such physical characteristics include, although not limited to, rigidity of the skirt and frame portion and thin-walled sample wells of the well and deck portion to permit optimal thermal transfer and biocompatibility. Methods of construction include forming the thin-well microplate as a unitary plate in a single, two-step process, wherein the skirt and frame portion is constructed of a suitable first material in a first step and the deck and frame portion is formed integral with the skirt and frame portion in a second step of a suitable second material. The unitary thin-well microplate plate is thereby formed of two materials for the required specific combination of physical and material properties. Another method of construction includes forming the unitary thin-well microplate in two separate manufacturing processes, wherein the skirt and frame portion is constructed of the first material in a first process and the deck and well portion constructed of the second material in a second process, and the skirt and frame portion and the well and deck portion are, thereafter, permanently joined by an adhesive system to form the unitary plate of the invention.

Claims

1. A thin-well microplate comprising:

a skirt and frame portion, constructed of a first material, having a top surface and a bottom surface having a plurality of holes arranged in a first array pattern extending from the top surface to the bottom surface, and walls of equal depth extending from the bottom surface;

a well and deck portion, constructed of a second material, joined with the top surface of the skirt and frame portion to form a unitary plate;

a plurality of sample wells integral with the well and deck portion arranged in the first array pattern such that the plurality of sample wells extend through the plurality of holes in the top surface and bottom surface of the skirt and frame portion.

2. The thin-well microplate of claim 1, wherein the first material imparts rigidity to the skirt and frame portion to allow use of automated equipment with the thin-well microplate.

3. The thin-well microplate of claim 1, wherein the second material forms the sample wells with thin walls having a thickness appropriate to allow adequate thermal transfer.

4. The thin-well microplate of claim 1, wherein the second material forms the sample wells with sufficient opacity to allow use of optical detection equipment with the thin-well microplate.

5. The thin-well microplate of claim 1, wherein the skirt and frame portion and the well and deck portion are formed of separate components and permanently joined to form the unitary plate.

6. The thin-well microplate of claim 1, wherein the well and deck portion is integrally formed with the top surface of the skirt and frame portion.

7. The thin-well microplate of claim 1, wherein the skirt and frame portion includes four walls forming a bottom of the skirt and frame portion opposite the top surface.

8. The thin-well microplate of claim 7, wherein the bottom has a length and width slightly larger than the length and width of the top surface.

9. The thin-well microplate of claim 1, further comprising a raised rim around an opening of each of the sample wells which is contiguous with an upper surface of the well and deck portion.

10. The thin-well microplate of claim 9, further comprising grooves between the raised rims of adjacent sample wells.

11. The thin-well microplate of claim 1, further comprising at least one indentation in each wall of the skirt and frame portion for engagement of automated equipment.

12. The thin-well microplate of claim 1, wherein an upper surface of the well and deck portion includes a plurality of interconnecting links, each interconnecting link joining at least two of the plurality of sample wells.

15. The thin-well microplate of claim 1, wherein the first material is a polymer resin.

16. The thin-well microplate of claim 1, wherein the first material is a filled polymer resin.

17. The thin-well microplate of claim 16, wherein the filled polymer resin is capable of withstanding a temperature of at least 100°

C.

18. The thin-well microplate of claim 16, wherein the filled polymer resin is glass-filled polypropylene.

19. The thin-well microplate of claim 1, wherein the second material is a polymer resin.

20. The thin-well microplate of claim 1, wherein the second material is an unfilled polymer resin.

21. The thin-well microplate of claim 20, wherein the unfilled polymer resin is capable of withstanding a temperature of at least 100°

C.

22. The thin-well microplate of claim 20, wherein the unfilled polymer resin is unfilled polypropylene.

13. A thin-well microplate comprising:

a skirt and frame portion, constructed of a first material, having a top surface and a bottom surface having a plurality of holes arranged in a first array pattern extending from the top surface to the bottom surface, and walls of equal depth extending from the bottom surface;

a plurality of sample wells, constructed of a second material, arranged in the first array pattern such that the plurality of sample wells extend through the plurality of holes in the top surface and bottom surface of the skirt and frame portion.

14. The thin-well microplate of claim 13, further comprising a plurality of interconnecting links, each interconnecting link joining at least two of the plurality of sample wells.