Chimeric gene for the transformation of plants

• US 6,362,396 B1
• Filed: 01/26/1998
• Issued: 03/26/2002
• Est. Priority Date: 03/05/1992
• Status: Expired due to Fees

• Current Assignee: Bayer SAS (Bayer AG)
• Original Assignee: Aventis Crop Science Company Limited
• Inventors: Freyssinet, Georges, Lebrun, Michel, Leroux, Bernard, Sailland, Alain, Gigot, Claude, Chaubet, Nicole
• Primary Examiner(s): Benzion, Gary
• Application Number:
  US09/013,520
• Time in Patent Office: 1,520 Days
• Field of Search: 800/295, 800/298, 536/23.6, 536/24.1, 536/23.2, 536/23.1, 435/69.1, 435/69.7
• US Class Current: 800/295
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 9/1092 3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Abstract

1) Chimeric gone for conferring to plants an increased tolerance to a herbicide. 2) It comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. 3) Production of glyphosate-tolerant plants.

Claims

1. A plant cell containing a chimeric gene, the chimeric gene encoding a transit peptide region comprising, in the direction of translation, at least one transit peptide of a plastid-localized enzyme, a partial sequence of an N-terminal mature part of a plastid-localized enzyme, and a second transit peptide of a plastid-localized enzyme.

2. The plant cell of claim 1, wherein the transit peptides and partial mature sequence are from sunflower or maize.

3. The plant cell of claim 2, wherein the chimeric gene further comprises a promoter region comprising at least one fragment of an Arabidopsis H3 or H4 histone gene promoter.

4. A plant which contains in its genome a chimeric gene encoding a transit peptide region comprising, in the direction of translation, at least one transit peptide of a plant gene which encodes a plastid-localized enzyme, a partial sequence of an N-terminal mature part of the plastid-localized enzyme, and a second transit peptide of a plastid-localized enzyme.

5. The plant of claim 4, wherein the chimeric gene further comprises a promoter region comprising at least one fragment of an Arabidopsis H3 or H4 histone gene promoter.