Methods of culturing and encapsulating pancreatic islet cells

  • US 6,365,385 B1
  • Filed: 12/01/1999
  • Issued: 04/02/2002
  • Est. Priority Date: 03/22/1999
  • Status: Expired due to Fees
First Claim
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1. A method of treating isolated pancreatic islet cells, comprising:

  • (a) culturing said cells in a medium containing at least one compound selected from the group consisting of an antioxidant, an anti-cytokine, an anti-endotoxin, and an antibiotic;

    then (b) microencapsulating said cells in a biocompatible microcapsule comprising a core and a semipermeable outer membrane, to provide a microcapsule containing living cells therein; and

    then (c) incubating said microcapsule containing living cells therein with a physiologically acceptable salt to increase the durability of the microcapsule, while retaining the physiological responsiveness of the living cells contained in the microcapsule to produce microcencapsulated islet cells exhibiting a weight gain of not more than 10 percent by weight over a period of one month in physiological saline solution at 37 degrees Celsius and exhibiting at least 150 percent basal insulin secretion in response to 16.7 milliMolar glucose challenge in Krebs-Ringer physiological solution at pH 7.4 after said period of one month.

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