Optical sensor for sensing multiple analytes

• US 6,379,969 B1
• Filed: 03/02/2000
• Issued: 04/30/2002
• Est. Priority Date: 03/02/2000
• Status: Expired due to Term

• Current Assignee: Agilent Technologies Europe BV (Agilent Technologies, Inc.)
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Curry, Bo, Mauze, Ganapati R.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/517,259
• Time in Patent Office: 789 Days
• Field of Search: 436/74, 436/68, 436/79, 436/95, 436/98, 436/163, 436/172, 422/82.05, 422/82.07, 422/82.08
• US Class Current: 436/68
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/147777 Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.]

Abstract

A device for analyzing simultaneously multiple analytes in a fluid of unknown composition. The device includes a plurality of sensors, a light source for providing light to shine on the sensors, light detectors, and a processor. The sensors are exposed to a sample of the fluid of unknown composition. The plurality of sensors includes groups of sensors, each group targeting a specific analyte and including one or more sensors that contain an analyte-specific chemical that interacts more specifically with one analyte than with some other analytes to be analyzed. Each sensor in each group has a different chemical interacting with the analyte to target it. The light source shines light on the sensors of the plurality of sensors to cause light interaction with the sensors. The differences in the sensors lead to differences in the light interaction. The light detectors detects the light interaction by the sensors. The processor analyzes the light interaction by the sensors to take into account interference in light interaction among the analytes, thereby determining the concentration of each of the analytes in the fluid.

Claims

1. A device for analyzing multiple analytes simultaneously in a fluid, comprising:

(a) a plurality of sensors each for exposing to a sample of the fluid, the plurality of sensors comprising;

groups of sensors, each group targeting a specific analyte, each group including one or more sensors including an analyte-specific chemical that interacts more specifically with one analyte than with some other analytes to be analyzed;

(b) light source for providing light to shine on the sensors of said plurality of sensors to cause light interaction with said sensors, wherein the differences in the sensors lead to differences in said light interaction;

(c) detectors for determining the light interaction by the sensors; and

(d) processor for analyzing the light interaction by the sensors to take into account interference in light interaction among the analytes, thereby determining the concentration of each of the analytes in the fluid.

2. A device according to claim 1 wherein at least some of the sensors each comprise a matrix in which the analyte of interest can penetrate, the analyte-specific chemical that chemically interacts specifically with the analyte, a fluorophore that fluoresces at a characteristic frequency, the fluorescence of said fluorophore being affected by the amount of analyte chemically interacting with the analyte-specific chemical.

3. A device according to claim 2 comprising a chemical indicator, wherein the chemical interaction of the analyte with the analyte-specific chemical can change absorption of the chemical indicator at the fluorescence frequency of the fluorophore.

4. A device according to claim 3 wherein in for at least some of the sensors the analyte-specific chemical is not the same as the chemical indicator and the amount of chemical interaction of the analyte-specific chemical with the analyte changes the chemical state of the chemical indicator to affect the absorption thereby at the fluorescence frequency of the fluorophore.

5. A device according to claim 2 wherein more than half of sensors in the plurality of sensors comprise one of a pH sensor and an oxygen sensor and wherein at least one of the groups include sensors having different chemicals interacting specifically with an analyte.

6. A device according to claim 5 comprising sensors that use a pH sensor or an oxygen sensor to determine the concentration of an analyte other than that of hydrogen ion or oxygen.

7. A device according to claim 2 comprising at least some sensors that can detect the concentration of at least five of the following:

hydrogen ion, carbon dioxide, oxygen, potassium ion, sodium ion, calcium ion, chloride ion, blood urea nitrogen (BUN), glucose, lactate, and creatinine.

8. A device according to claim 2 wherein the same light source is used to provide light to shine on the plurality of sensors.

9. A device according to claim 2 comprising light outlets each for directing light at a sensor and said outlet is surrounded by detectors.

10. A device according to claim 2 wherein the light interaction includes luminescence decay of fluorescence.

11. A device according to claim 2 wherein the light source includes one of light emitting diode, vertical cavity surface emitting laser, and laser diodes.

12. A device according to claim 2 wherein the processor analyzes the light interaction of the sensors using a matrix algorithm.

13. A device for analyzing simultaneously multiple analytes in a fluid, comprising:

(a) a plurality of sensors each for exposing to a sample of the fluid, the plurality of sensors comprising;

groups of sensors, each group having one or more sensors each having an analyte-specific chemical that interacts more specifically with one analyte than with some other analytes to be analyzed, wherein at least half of the sensors for sensing analytes other than hydrogen ions or oxygen include a pH sensor or an oxygen sensor;

(b) light source for providing light to shine on the sensors of said plurality of sensors to cause light interaction with said sensors, wherein the differences in the sensors lead to differences in said light interaction, wherein at least some of the sensors specific for different analytes receive light of the same frequency profile;

(c) detectors for determining the light interaction by the sensors; and

(d) processor for analyzing the light interaction by the sensors to take into account interference in light interaction among the analytes using a matrix algorithm, thereby determining the concentration of each of the analytes in the fluid.

14. A method for analyzing simultaneously multiple analytes in a fluid, comprising:

(a) exposing a plurality of sensors each to a sample of the fluid, the plurality of sensors having groups of sensors, each group having one or more sensors each including an analyte-specific chemical that interacts more specifically with one analyte than with some other analytes to be analyzed; and

(b) shining light on the sensors of said plurality of sensors to cause light interaction with said sensors, wherein the differences in the sensors lead to differences in said light interaction;

(c) detecting the light interaction by the sensors;

(d) mathematically analyzing the light interaction by the sensors to take into account interference in light interaction among the analytes, thereby determining the concentration of each of the analytes in the fluid.

15. A method according to claim 14 comprising allowing an analyte of interest in the fluid to pass through a matrix in a sensor to chemically interact with the analyte-specific chemical, thereby affecting the fluorescence of a fluorophore in the sensor to indicate the concentration of the analyte in the fluid.

16. A method according to claim 15 comprising using a pH sensor or an oxygen sensor to determine the concentration of an analyte other than that of hydrogen ion or oxygen, and wherein a group has sensors with different chemicals interacting specifically with an analyte.

17. A method according to claim 15 comprising determining the concentration of at least the following:

hydrogen ion, carbon dioxide, oxygen, potassium.

18. A method according to claim 15 comprising detecting the concentration of at least five of the following:

hydrogen ion, carbon dioxide, oxygen, potassium ion, sodium ion, calcium ion, chloride ion, blood urea nitrogen (BUN), glucose, lactate, and creatinine.

19. A method according to claim 14 wherein the chemical interaction of the analyte with the analyte-specific chemical affects absorption of a chemical indicator at the fluorescence frequency of the fluorophore.

20. A method according to claim 14 comprising using the same light source is used to provide light to shine on the plurality of sensors.

21. A method according to claim 14 comprising directing light from a light outlet at a sensor and detecting fluorescence from the sensor by detectors surrounding said light outlet.

22. A method according to claim 14 wherein the light interaction includes luminescence decay of fluorescence.

23. A method according to claim 14 comprising analyzing the light interaction of the sensors using a matrix algorithm to take into account the interference of light interaction among the sensors.

24. An method of making a device for analyzing simultaneously multiple analytes in a fluid, comprising:

(a) making a plurality of sensors each for exposing to a sample of the fluid, the step of making the plurality of sensors comprising making groups of sensors wherein each group having one or more sensors including an analyte-specific chemical that interacts more specifically with one analyte than with some other analytes to be analyzed;

(b) positioning a light source for providing light to shine on the sensors of said plurality of sensors to cause light interaction with said sensors, wherein the differences in the sensors lead to differences in said light interaction;

(c) positioning detectors for determining the light interaction by the sensors; and

(d) electrically connecting the detectors to a processor for analyzing the light interaction by the sensors to take into account interference in light interaction among the analytes, thereby determining the concentration of each of the analytes in the fluid.