Method of producing transgenic maize using direct transformation of commercially important genotypes

  • US 6,403,865 B1
  • Filed: 05/10/1995
  • Issued: 06/11/2002
  • Est. Priority Date: 08/24/1990
  • Status: Expired due to Term
First Claim
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1. A fertile transgenic maize plant comprising:

  • a foreign DNA sequence encoding a Bacillus thuringiensis insecticidal protein toxic to European corn borer stably incorporated into the plant'"'"'s genome, the foreign DNA comprising a nucleic acid coding sequence modified from the nucleic acid coding sequence of the native Bacillus thuringiensis gene encoding the insecticidal protein to increase expression of the insecticidal protein in the transgenic plant;

    wherein the transgenic plant expresses the insecticidal protein in plant leaf tissue at greater than about 1-5 ng insecticidal protein per mg soluble leaf protein, and the leaf tissue causes mortality to European corn borer.

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